Mechanism of cytosol phospholipase c and sphingomyelinase-induced lysosome destabilization
文献类型：期刊论文
| 作者 | Wang, Xiang; Zhao, Hui-Fang; Zhang, Guo-Hang |
| 刊名 | Biochimie
 |
| 出版日期 | 2006-07-01 |
| 卷号 | 88期号:7页码:913-922 |
| ISSN号 | 0300-9084 |
| 关键词 | Lysosome Phospholipase c Sphingomyelinase Calcium ion Potassium ion Proton |
| DOI | 10.1016/j.biochi.2006.02.005 |
| 通讯作者 | Zhang, guo-hang(zhanggj@sun5.ibp.ac.cn) |
| 英文摘要 | Lysosomal disintegration may cause apoptosis, necrosis and some diseases. however, mechanisms for these events are still unclear. in this study, we measured lysosomal p-hexosaminidase free activity, membrane potential and intralysosomal ph. the results revealed that the cytosolic extracts of rat hepatocytes could increase the lysosomal permeability to both potassium ions and protons, and osmotically destabilize lysosomes via k+/h+ exchange. the effects of cytosol on lysosomes could be completely abolished by d609, which inhibited both phospholipase c and sphingomyelinase, and partly prevented by sphingomyelinase inhibitor ara-amp, but not by the inhibitors of pla(2). moreover, purified phospholipase c could destabilize the lysosomes while phospholipase a(2) and phospholipase d did not produce such effects. the cytosolic phospho-lipases hydrolyzed lysosomal membrane phospholipids by 50%, which could be prevented by d609. disintegration of the cytosol-treated lysosomes biphasically depended on the cytosolic [ca2+]. the cytosol did not disintegrate lysosomes below 100 nm or above 10 mu m cytosolic [ca2+], but markedly destabilized lysosomes at about 340 nm [ca2+]. the results suggest that cytosolic phospholipase c and sphingomyelinase may be responsible for the alterations in lysosomal stability by increasing the ion permeability. (c) 2006 elsevier sas. all rights reserved. |
| WOS关键词 | RAT-LIVER LYSOSOMES ; CELL-DEATH ; OSMOTIC SENSITIVITY ; PROTON PUMP ; DNA-DAMAGE ; APOPTOSIS ; MEMBRANE ; INVOLVEMENT ; ACTIVATION ; TRANSLOCATION |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| 出版者 | ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER |
| WOS记录号 | WOS:000240167100019 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378581 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhang, Guo-Hang |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Univ Sci & Technol Beijing, Beijing 100083, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiang,Zhao, Hui-Fang,Zhang, Guo-Hang. Mechanism of cytosol phospholipase c and sphingomyelinase-induced lysosome destabilization[J]. Biochimie,2006,88(7):913-922. |
| APA | Wang, Xiang,Zhao, Hui-Fang,&Zhang, Guo-Hang.(2006).Mechanism of cytosol phospholipase c and sphingomyelinase-induced lysosome destabilization.Biochimie,88(7),913-922. |
| MLA | Wang, Xiang,et al."Mechanism of cytosol phospholipase c and sphingomyelinase-induced lysosome destabilization".Biochimie 88.7(2006):913-922. |
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来源：中国科学院大学
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