Over-expression and characterization of the recombinant small heat shock protein from pyrococcus furiosus
文献类型：期刊论文
| 作者 | Chen, Huayou; Chu, Zhongmei; Zhang, Yi; Yang, Shengli |
| 刊名 | Biotechnology letters
 |
| 出版日期 | 2006-07-01 |
| 卷号 | 28期号:14页码:1089-1094 |
| 关键词 | Chaperone Pyrococcus furiosus Small heat shock protein |
| ISSN号 | 0141-5492 |
| DOI | 10.1007/s10529-006-9058-y |
| 通讯作者 | Zhang, yi(yzhang@sibs.ac.cn) |
| 英文摘要 | The gene encoding a small heat shock protein (shsp) from pyrococcus furiosus was redesigned and chemically synthesized by using bacteria-preferred codons. the gene product was over-expressed in escherichia coli bl21(de)(3) and purified to homogeneity. in the presence of this protein, the activities of taq dna polymerase, dna restriction endonuclease hindiii and lysozyme were protected at elevated temperature, and also, thermal aggregation of lysozyme was prevented by this purified recombinant shsp. |
| WOS关键词 | HYPERTHERMOPHILIC ARCHAEON ; MULTICHAPERONE NETWORK ; ESCHERICHIA-COLI |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000238829600006 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378628 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhang, Yi |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai 200233, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Huayou,Chu, Zhongmei,Zhang, Yi,et al. Over-expression and characterization of the recombinant small heat shock protein from pyrococcus furiosus[J]. Biotechnology letters,2006,28(14):1089-1094. |
| APA | Chen, Huayou,Chu, Zhongmei,Zhang, Yi,&Yang, Shengli.(2006).Over-expression and characterization of the recombinant small heat shock protein from pyrococcus furiosus.Biotechnology letters,28(14),1089-1094. |
| MLA | Chen, Huayou,et al."Over-expression and characterization of the recombinant small heat shock protein from pyrococcus furiosus".Biotechnology letters 28.14(2006):1089-1094. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。