Expression, purification, crystallization and preliminary crystallographic analysis of the human intracellular chloride channel protein clic4
文献类型:期刊论文
作者 | Li, DF; Li, YF; Huang, QH; Zhang, Y; Wang, DC |
刊名 | Protein and peptide letters
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出版日期 | 2006 |
卷号 | 13期号:6页码:641-643 |
关键词 | Clic4 Human chloride intracellular channel protein Purification Crystallization Preliminary crystallographic studies |
ISSN号 | 0929-8665 |
通讯作者 | Wang, dc() |
英文摘要 | The human chloride intracellular channel protein clic4 has been crystallized by the hanging-drop vapour-diffusion technique using trisodium citrate as the precipitant. the best crystals were obtained by the microseeding method. the crystals diffracted to 2.2 angstrom resolution and were found to belong to space group p 12 1, with unit-cell parameters a 73.19, b=86.05, c=73.38 angstrom, p = 112.99 degrees and three molecule per asymmetric unit. |
WOS关键词 | MOLECULAR-CLONING ; P64 ; HOMOLOG ; NEURONS ; P53 |
WOS研究方向 | Biochemistry & Molecular Biology |
WOS类目 | Biochemistry & Molecular Biology |
语种 | 英语 |
WOS记录号 | WOS:000238215700019 |
出版者 | BENTHAM SCIENCE PUBL LTD |
URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378631 |
专题 | 中国科学院大学 |
通讯作者 | Wang, DC |
作者单位 | 1.Chinese Acad Sci, Inst Biophys, Ctr Struct & Mol Biol, Beijing 100101, Peoples R China 2.Shanghai Med Univ 2, Rui Jin Hosp, Shanghai Inst Hematol, Shanghai 200025, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
推荐引用方式 GB/T 7714 | Li, DF,Li, YF,Huang, QH,et al. Expression, purification, crystallization and preliminary crystallographic analysis of the human intracellular chloride channel protein clic4[J]. Protein and peptide letters,2006,13(6):641-643. |
APA | Li, DF,Li, YF,Huang, QH,Zhang, Y,&Wang, DC.(2006).Expression, purification, crystallization and preliminary crystallographic analysis of the human intracellular chloride channel protein clic4.Protein and peptide letters,13(6),641-643. |
MLA | Li, DF,et al."Expression, purification, crystallization and preliminary crystallographic analysis of the human intracellular chloride channel protein clic4".Protein and peptide letters 13.6(2006):641-643. |
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来源:中国科学院大学
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