Recognition of cell surface proteins by high-density phage antibody chip
文献类型：期刊论文
| 作者 | Cen Xiao-Dong; Wang Wen-Juan; Zhao Xin-Sheng; Wang Xuan; Shen Ti; Tan Tao-Chao; Bi Qun; Zhu Sheng-Geng |
| 刊名 | Acta physico-chimica sinica
 |
| 出版日期 | 2006-07-01 |
| 卷号 | 22期号:7页码:777-779 |
| 关键词 | Phage antibody library Pviii display system High-density phage antibody chip Leukemia Leukocytic surface protein |
| ISSN号 | 1000-6818 |
| DOI | 10.3866/pku.whxb20060702 |
| 通讯作者 | Zhu sheng-geng(zhusg@pku.edu.cn) |
| 英文摘要 | A large human phage antibody library was subjected for panning with leukocytes from healthy donors and leukemia patients to select for specific antibodies against leukocytic surface proteins. recombinant phage particles displaying multiple antibody fragments via pviii display system were used to prepare high-density phage antibody chips. leukocyte lysate samples from 3 healthy donors and 3 leukemia patients were labeled with cy3 fluorescence dye and reacted with the microarrays on the epoxy substrates. eight sets of specific spots were found to have significantly distinct fluorescence intensities between the two groups of fluorescence profiles. it was demonstrated that phage antibody chips could be used for the recognition of cellular surface proteins. |
| WOS关键词 | MICROARRAY ; DISPLAY ; BINDING ; LIBRARY ; CANCER ; SYSTEM ; ARRAY |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Physical |
| 语种 | 英语 |
| WOS记录号 | WOS:000239177800002 |
| 出版者 | PEKING UNIV PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378733 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhu Sheng-Geng |
| 作者单位 | 1.Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China 2.Peking Univ, Coll Chem & Chem Engn, Dept Biol Chem, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100871, Peoples R China 3.Beijing Union Med Coll Hosp, Beijing 100730, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cen Xiao-Dong,Wang Wen-Juan,Zhao Xin-Sheng,et al. Recognition of cell surface proteins by high-density phage antibody chip[J]. Acta physico-chimica sinica,2006,22(7):777-779. |
| APA | Cen Xiao-Dong.,Wang Wen-Juan.,Zhao Xin-Sheng.,Wang Xuan.,Shen Ti.,...&Zhu Sheng-Geng.(2006).Recognition of cell surface proteins by high-density phage antibody chip.Acta physico-chimica sinica,22(7),777-779. |
| MLA | Cen Xiao-Dong,et al."Recognition of cell surface proteins by high-density phage antibody chip".Acta physico-chimica sinica 22.7(2006):777-779. |
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来源：中国科学院大学
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