Rapid separation and determination of resibufogenin and cinobufagin in toad venom and liushen tablet by beta-cyclodextrin modified micellar electrokinetic chromatography
文献类型：期刊论文
| 作者 | Zhao, YF; Luo, XP; Ming, YF; Chen, LR; Li, YM |
| 刊名 | Journal of pharmaceutical and biomedical analysis
 |
| 出版日期 | 2006-04-11 |
| 卷号 | 41期号:1页码:124-128 |
| 关键词 | Micellar electrokinetic chromatography Beta-cyclodextrin Resibufogenin Cinobufagin Toad venom |
| ISSN号 | 0731-7085 |
| DOI | 10.1016/j.jpba.2005.10.046 |
| 通讯作者 | Chen, lr() |
| 英文摘要 | A rapid cyclodextrin modified micellar electrokinetic chromatography (cd-mekc) method was proposed for the determination of resibufogenin and cinobufagin in the chinese herbal extracts from toad venom and its medicinal preparation (liushen tablet). the two components have the close structural similarity and similar hydrophobicity, which result in poor resolution in normal mekc. the addition of neutral beta-cd to the mekc system was found to improve the separation of the studied compounds. the effects of several cd-mekc parameters on the resolutions were evaluated systematically. based on the investigation, a background electrolyte solution consisting of 10 mm borate buffer adjusted to ph 8.5, 40 mm sodium dodecyl sulfate (sds), 12 mm beta-cd and 10% (v/v) of methanol was found to be optimal conditions for the fast separation. the contents of resibufogenin and cinobufagin were successfully determined within 5 min, with satisfactory repeatability and recovery. (c) 2005 elsevier b.v. all rights reserved. |
| WOS关键词 | TRADITIONAL CHINESE MEDICINE ; LIQUID-CHROMATOGRAPHY ; CHIRAL SEPARATION ; LEUKEMIA-CELLS ; SHEN-WAN ; CHAN-SU ; BUFADIENOLIDES ; DIFFERENTIATION ; ENANTIOMERS ; BUFALIN |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| WOS类目 | Chemistry, Analytical ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000236655100016 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378742 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chen, LR |
| 作者单位 | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, YF,Luo, XP,Ming, YF,et al. Rapid separation and determination of resibufogenin and cinobufagin in toad venom and liushen tablet by beta-cyclodextrin modified micellar electrokinetic chromatography[J]. Journal of pharmaceutical and biomedical analysis,2006,41(1):124-128. |
| APA | Zhao, YF,Luo, XP,Ming, YF,Chen, LR,&Li, YM.(2006).Rapid separation and determination of resibufogenin and cinobufagin in toad venom and liushen tablet by beta-cyclodextrin modified micellar electrokinetic chromatography.Journal of pharmaceutical and biomedical analysis,41(1),124-128. |
| MLA | Zhao, YF,et al."Rapid separation and determination of resibufogenin and cinobufagin in toad venom and liushen tablet by beta-cyclodextrin modified micellar electrokinetic chromatography".Journal of pharmaceutical and biomedical analysis 41.1(2006):124-128. |
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来源：中国科学院大学
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