中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Genetic diversity of populus cathayana rehd populations in southwestern china revealed by issr markers

文献类型:期刊论文

作者Lu, ZX; Wang, YH; Peng, YH; Korpelainen, H; Li, CY
刊名Plant science
出版日期2006-02-01
卷号170期号:2页码:407-412
关键词Qinghai-tibetan plateau Populus cathayana rehd Genetic variation Issr
ISSN号0168-9452
DOI10.1016/j.plantsci.2005.09.009
通讯作者Li, cy()
英文摘要As a native species to china, populus cathayana rehd is well-adapted to the wet and cold environments where it occurs. it is considered to be an important reforestation species in western china. in the present study, we surveyed the level of genetic variation and the pattern of genetic structure in seven natural populations of r cathayana, originating from the southeastern qinghai-tibetan plateau of china, by using issr (inter-simple sequence repeats) markers. based on eight primers, 158 clear and reproducible dna fragments were generated, of which 156 (98.7%) were polymorphic. the average value of nei's gene diversity (h) equaled 0.331. the coefficient of genetic differentiation (g(st)) equaled 0.477, which means that 47.7% of the total molecular variance existed among populations. such a high level of divergence present among populations may be caused by the complex topography and variable climatic conditions present in the southeastern qinghai-tibetan plateau which effectively restrict gene flow. moreover, there is a lack of significant association between genetic and geographical distances (r = 0.3122, p > 0.05) in the populations of r cathayana. the application of a novel method, which combines geographical coordinates and genetic differentiation to detect barriers for gene flow, allowed us to identify two zones of lowered gene flow. the issr method used in the present genetic fingerprinting-based study proved to be an effective high-resolution tool for identifying patterns of genetic variation present in forest trees. in the case of p. cathayana, such information is greatly needed for planning conservation genetic actions to ensure the maintenance of appropriate levels of genetic variation. (c) 2005 elsevier ireland ltd. all rights reserved.
WOS关键词SEQUENCE REPEAT ANALYSIS ; POPLAR POPULUS ; BLACK POPLAR ; GENOME ; RAPD ; IDENTIFICATION ; ALLOZYME ; CITRUS ; DNA
WOS研究方向Biochemistry & Molecular Biology ; Plant Sciences
WOS类目Biochemistry & Molecular Biology ; Plant Sciences
语种英语
WOS记录号WOS:000234732700024
出版者ELSEVIER IRELAND LTD
URI标识http://www.irgrid.ac.cn/handle/1471x/2378777
专题中国科学院大学
通讯作者Li, CY
作者单位1.Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Univ Helsinki, Dept Appl Biol, FI-00014 Helsinki, Finland
推荐引用方式
GB/T 7714
Lu, ZX,Wang, YH,Peng, YH,et al. Genetic diversity of populus cathayana rehd populations in southwestern china revealed by issr markers[J]. Plant science,2006,170(2):407-412.
APA Lu, ZX,Wang, YH,Peng, YH,Korpelainen, H,&Li, CY.(2006).Genetic diversity of populus cathayana rehd populations in southwestern china revealed by issr markers.Plant science,170(2),407-412.
MLA Lu, ZX,et al."Genetic diversity of populus cathayana rehd populations in southwestern china revealed by issr markers".Plant science 170.2(2006):407-412.

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来源:中国科学院大学

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