Cloning, e-coli expression and molecular analysis of amorpha-4,11-diene synthase from a high-yield strain of artemisia annua l.
文献类型：期刊论文
| 作者 | Li, Zhen-Qiu; Liu, Yan; Liu, Ben-Ye; Wang, Hong; Ye, He-Chun; Li, Guo-Feng |
| 刊名 | Journal of integrative plant biology
 |
| 出版日期 | 2006-12-01 |
| 卷号 | 48期号:12页码:1486-1492 |
| 关键词 | Amorpha-4,11-diene synthase Artemisia annua E. coli expression Molecular analysis |
| ISSN号 | 1672-9072 |
| DOI | 10.1111/j.1672-9072.2006.00381.x |
| 通讯作者 | Ye, he-chun(hcye@ibcas.ac.cn) |
| 英文摘要 | Increasing demand of arternisinin in the treatment of malaria has placed substantial stress on the total artemisinin supplies world-wide, so more attention has; been paid to increasing the content of arternisinin in the artemisia annua l. plant. in this study, amorpha-4,11-diene synthase (ads) cdna (ads1) and genomics gene (gads1) were cloned from a high-yield a. annua strain 001. the activity of ads1 was confirmed by heterogeneous overexpression of adsl and in vitro enzymatic incubation. reverse transcript-polymerase chain reaction results demonstrated that adsl expressed in leaves, flowers and young stems, but not in roots. this organ-specific expression pattern of adsl is consistent with that of artemisinin accumulation in the plant. the gads1 has a complex organization including seven exons and six introns, and belongs to class iii terpene synthase. dna gel blotting revealed that the ads gene has at least four copies in the genome of strain 001. the higher copy numbers might be one of the reasons for its high artemisinin content. |
| WOS关键词 | ANTIMALARIAL-DRUG ; CDNA ISOLATION ; BIOSYNTHESIS ; (E)-BETA-FARNESENE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS类目 | Biochemistry & Molecular Biology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000242845700014 |
| 出版者 | BLACKWELL PUBLISHING |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378781 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Ye, He-Chun |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Photosynth & Environm Mol Physiol, Inst Bot, Beijing 100093, Peoples R China 2.Hebei Univ, Coll Life Sci, Baoding 071002, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Zhen-Qiu,Liu, Yan,Liu, Ben-Ye,et al. Cloning, e-coli expression and molecular analysis of amorpha-4,11-diene synthase from a high-yield strain of artemisia annua l.[J]. Journal of integrative plant biology,2006,48(12):1486-1492. |
| APA | Li, Zhen-Qiu,Liu, Yan,Liu, Ben-Ye,Wang, Hong,Ye, He-Chun,&Li, Guo-Feng.(2006).Cloning, e-coli expression and molecular analysis of amorpha-4,11-diene synthase from a high-yield strain of artemisia annua l..Journal of integrative plant biology,48(12),1486-1492. |
| MLA | Li, Zhen-Qiu,et al."Cloning, e-coli expression and molecular analysis of amorpha-4,11-diene synthase from a high-yield strain of artemisia annua l.".Journal of integrative plant biology 48.12(2006):1486-1492. |
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来源：中国科学院大学
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