Thioredoxin-mediated negative autoregulation of peroxisome proliferator-activated receptor alpha transcriptional activity
文献类型：期刊论文
| 作者 | Liu, GH; Qu, J; Shen, X |
| 刊名 | Molecular biology of the cell
 |
| 出版日期 | 2006-04-01 |
| 卷号 | 17期号:4页码:1822-1833 |
| ISSN号 | 1059-1524 |
| DOI | 10.1091/mbc.e05-10-0979 |
| 通讯作者 | Shen, x() |
| 英文摘要 | Ppar alpha, a member of the nuclear receptor superfamily, and thioredoxin, a critical redox-regulator in cells, were found to form a negative feedback loop, which autoregulates transcriptional activity of ppara. thioredoxin was identified as a target gene of ppara. activation of ppara leads to increase of thioredoxin expression as well as its translocation from cytoplasm to nucleus, whereas ectopic overexpression of thioredoxin in the nucleus dramatically inhibited both constitutive and ligand-dependent ppara activation. as ppar alpha-target genes, the expression of muscle carnitine palmitoyltransferase 1, medium chain acyl coa dehydrogenase, and apolipoprotein a-i were significantly down-regulated by nucleus-targeted thioredoxin at transcriptional or protein level. the suppression of ppara transcriptional activity by trx could be enhanced by overexpression of thioredoxin reductase or knockdown of thioredoxin-interacting protein, but abrogated by mutating the redox-active sites of thioredoxin. mammalian one-hybrid assays showed that thioredoxin inhibited ppara activity by modulating its af-1 transactivation domain. it was also demonstrated by electrophoretic mobility-shift assay that thioredoxin inhibited the binding of ppara to the ppar-response element. together, it is speculated that the reported negative-feedback loop may be essential for maintaining the homeostasis of ppara activity. |
| WOS关键词 | NF-KAPPA-B ; PPAR-ALPHA ; INTERACTING PROTEIN ; RESPONSIVE ELEMENT ; ENDOTHELIAL-CELLS ; OXIDATIVE STRESS ; REDOX REGULATION ; GENE-EXPRESSION ; AP-1 ACTIVITY ; OXIDASE GENE |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| 语种 | 英语 |
| 出版者 | AMER SOC CELL BIOLOGY |
| WOS记录号 | WOS:000236657900030 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378789 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Shen, X |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100101, Peoples R China 3.Shanghai Univ Trad Chinese Med, E Inst Shanghai Univ, Div Nitr Oxide & Inflammatory Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, GH,Qu, J,Shen, X. Thioredoxin-mediated negative autoregulation of peroxisome proliferator-activated receptor alpha transcriptional activity[J]. Molecular biology of the cell,2006,17(4):1822-1833. |
| APA | Liu, GH,Qu, J,&Shen, X.(2006).Thioredoxin-mediated negative autoregulation of peroxisome proliferator-activated receptor alpha transcriptional activity.Molecular biology of the cell,17(4),1822-1833. |
| MLA | Liu, GH,et al."Thioredoxin-mediated negative autoregulation of peroxisome proliferator-activated receptor alpha transcriptional activity".Molecular biology of the cell 17.4(2006):1822-1833. |
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来源：中国科学院大学
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