An amperometric bienzyme biosensor for rapid measurement of alanine aminotransferase in whole blood
文献类型：期刊论文
| 作者 | Li, Huaqing; Guo, Zonghui; Wang, Hui; Cui, Dafu; Cai, Xinxia |
| 刊名 | Sensors and actuators b-chemical
 |
| 出版日期 | 2006-12-07 |
| 卷号 | 119期号:2页码:419-424 |
| 关键词 | Redox hydrogel Amperometric Biosensor Alanine aminotransferase L-glutamate oxidase Horseradish peroxidase |
| ISSN号 | 0925-4005 |
| DOI | 10.1016/j.snb.2005.12.041 |
| 通讯作者 | Cai, xinxia(xxcai@mail.ie.ac.cn) |
| 英文摘要 | A two-electrode configuration has been fabricated by depositing a gold film on a polyethylene terephthalate (pet) substrate. the integrated reference electrode was obtained by screen-printing commercial silver and silver chloride paste on the gold electrode. the working electrode was modified by drop-coating a redox polymer of poly(4-vinylpyrodine) complexed with osmium (2,2'-bipyrridine)(2). the bienzymes (horseradish peroxidase and l-glutamate oxidase) were immobilized in the redox hydrogel matrices by the poly (ethylene glycol) diglycidyl ether (pegdge) crosslinker. the optimized biosensor was used for the amperometric measurement of l-glutamate in a stirred solution at 0 mv versus ag vertical bar agcl. a linear calibration graph was obtained in the concentration range of l-glutamate from 1 to 500 mu m (linear regression coefficient = 0.99) with a steady-state response time of less than 10 s. the sensors were characterized by a sensitivity of 55.3 mu a mm(-1) cm(-2) with an operational stability of more than 90% of its original activity over a period of 1 month. the alanine aminotransferase in real human serum and whole blood samples was analyzed subsequently with this sensor method and compared to the clinical method with satisfactory results. (c) 2006 elsevier b.v. all rights reserved. |
| WOS关键词 | GLUTAMATE-PYRUVATE TRANSAMINASE ; COMPLEX-BILAYER MEMBRANE ; REFERENCE ELECTRODES ; OXIDASE ; SENSOR ; SERUM |
| WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| WOS类目 | Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation |
| 语种 | 英语 |
| WOS记录号 | WOS:000241197600011 |
| 出版者 | ELSEVIER SCIENCE SA |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378793 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Cai, Xinxia |
| 作者单位 | 1.Chinese Acad Sci, Inst Elect, State Key Lab Transducer Technol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Huaqing,Guo, Zonghui,Wang, Hui,et al. An amperometric bienzyme biosensor for rapid measurement of alanine aminotransferase in whole blood[J]. Sensors and actuators b-chemical,2006,119(2):419-424. |
| APA | Li, Huaqing,Guo, Zonghui,Wang, Hui,Cui, Dafu,&Cai, Xinxia.(2006).An amperometric bienzyme biosensor for rapid measurement of alanine aminotransferase in whole blood.Sensors and actuators b-chemical,119(2),419-424. |
| MLA | Li, Huaqing,et al."An amperometric bienzyme biosensor for rapid measurement of alanine aminotransferase in whole blood".Sensors and actuators b-chemical 119.2(2006):419-424. |
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来源：中国科学院大学
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