中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Effect of c-terminal truncation on the molecular chaperone function and dimerization of escherichia coli trigger factor

文献类型:期刊论文

作者Zeng, Li-Ling; Yu, Ling; Li, Zhen-Yu; Perrett, Sarah; Zhou, Jun-Mei
刊名Biochimie
出版日期2006-06-01
卷号88期号:6页码:613-619
关键词Chaperone Co-expression C-terminal truncation Dimerization Cross-linking Trigger factor
ISSN号0300-9084
DOI10.1016/j.biochi.2005.11.006
通讯作者Zhou, jun-mei(zhoujm@sun5.ibp.ac.cn)
英文摘要To examine the role of the c-terminal domain in the chaperone function of trigger factor (tf), a number of truncation mutants were constructed, namely: tf419, tf389, tf380, tf360, tf344, and tf251, in which the c-terminal 13, 43, 52, 72, 88 residues or the entire c-domain were deleted, respectively. co-expression of mutant chicken adenylate kinase (ak) with tf and the c-terminal truncation mutants was achieved using a plasmid pbvat that allows expression of tf and ak from a single plasmid. the results show that truncation of the c-terminus of tf has only minor effect on its ability to assist ak refolding in vivo. further, ribosome-binding experiments indicate that c-terminal truncation mutants can still bind to the ribosome and the presence of the c-terminus may in fact lower the affinity of tf for the ribosome in vivo. this indicates that the c-domain of trigger factor may not be essential for the ribosome-associated molecular chaperone function of tf. however, the purified tf c-terminal truncation mutants had a dramatically reduced ability to assist rabbit muscle gapdh refolding in vitro and a reduced tendency to dimerize. this shows that the structural integrity of the c-terminus contributes to both the chaperone function of tf and the stability of the dimeric form. (c) 2005 elsevier sas. all rights reserved.
WOS关键词CIS/TRANS ISOMERASE ACTIVITY ; ADENYLATE KINASE ; D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE ; GUANIDINE-HYDROCHLORIDE ; PROLYL ISOMERASE ; DOMAIN ; BINDING ; PROTEIN ; RIBOSOME ; SITE
WOS研究方向Biochemistry & Molecular Biology
WOS类目Biochemistry & Molecular Biology
语种英语
WOS记录号WOS:000239617600005
出版者ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
URI标识http://www.irgrid.ac.cn/handle/1471x/2378813
专题中国科学院大学
通讯作者Zhou, Jun-Mei
作者单位1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714
Zeng, Li-Ling,Yu, Ling,Li, Zhen-Yu,et al. Effect of c-terminal truncation on the molecular chaperone function and dimerization of escherichia coli trigger factor[J]. Biochimie,2006,88(6):613-619.
APA Zeng, Li-Ling,Yu, Ling,Li, Zhen-Yu,Perrett, Sarah,&Zhou, Jun-Mei.(2006).Effect of c-terminal truncation on the molecular chaperone function and dimerization of escherichia coli trigger factor.Biochimie,88(6),613-619.
MLA Zeng, Li-Ling,et al."Effect of c-terminal truncation on the molecular chaperone function and dimerization of escherichia coli trigger factor".Biochimie 88.6(2006):613-619.

入库方式: iSwitch采集

来源:中国科学院大学

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。