Effect of c-terminal truncation on the molecular chaperone function and dimerization of escherichia coli trigger factor
文献类型：期刊论文
| 作者 | Zeng, Li-Ling; Yu, Ling; Li, Zhen-Yu; Perrett, Sarah; Zhou, Jun-Mei |
| 刊名 | Biochimie
 |
| 出版日期 | 2006-06-01 |
| 卷号 | 88期号:6页码:613-619 |
| 关键词 | Chaperone Co-expression C-terminal truncation Dimerization Cross-linking Trigger factor |
| ISSN号 | 0300-9084 |
| DOI | 10.1016/j.biochi.2005.11.006 |
| 通讯作者 | Zhou, jun-mei(zhoujm@sun5.ibp.ac.cn) |
| 英文摘要 | To examine the role of the c-terminal domain in the chaperone function of trigger factor (tf), a number of truncation mutants were constructed, namely: tf419, tf389, tf380, tf360, tf344, and tf251, in which the c-terminal 13, 43, 52, 72, 88 residues or the entire c-domain were deleted, respectively. co-expression of mutant chicken adenylate kinase (ak) with tf and the c-terminal truncation mutants was achieved using a plasmid pbvat that allows expression of tf and ak from a single plasmid. the results show that truncation of the c-terminus of tf has only minor effect on its ability to assist ak refolding in vivo. further, ribosome-binding experiments indicate that c-terminal truncation mutants can still bind to the ribosome and the presence of the c-terminus may in fact lower the affinity of tf for the ribosome in vivo. this indicates that the c-domain of trigger factor may not be essential for the ribosome-associated molecular chaperone function of tf. however, the purified tf c-terminal truncation mutants had a dramatically reduced ability to assist rabbit muscle gapdh refolding in vitro and a reduced tendency to dimerize. this shows that the structural integrity of the c-terminus contributes to both the chaperone function of tf and the stability of the dimeric form. (c) 2005 elsevier sas. all rights reserved. |
| WOS关键词 | CIS/TRANS ISOMERASE ACTIVITY ; ADENYLATE KINASE ; D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE ; GUANIDINE-HYDROCHLORIDE ; PROLYL ISOMERASE ; DOMAIN ; BINDING ; PROTEIN ; RIBOSOME ; SITE |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000239617600005 |
| 出版者 | ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378813 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhou, Jun-Mei |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zeng, Li-Ling,Yu, Ling,Li, Zhen-Yu,et al. Effect of c-terminal truncation on the molecular chaperone function and dimerization of escherichia coli trigger factor[J]. Biochimie,2006,88(6):613-619. |
| APA | Zeng, Li-Ling,Yu, Ling,Li, Zhen-Yu,Perrett, Sarah,&Zhou, Jun-Mei.(2006).Effect of c-terminal truncation on the molecular chaperone function and dimerization of escherichia coli trigger factor.Biochimie,88(6),613-619. |
| MLA | Zeng, Li-Ling,et al."Effect of c-terminal truncation on the molecular chaperone function and dimerization of escherichia coli trigger factor".Biochimie 88.6(2006):613-619. |
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来源：中国科学院大学
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