Expression and purification of a putative tumor suppressor gene pp5715 in e-coli with growth inhibition of hepatocellular carcinoma cells
文献类型：期刊论文
| 作者 | Ma, WZ; Han, W; Qin, WX; Wan, DF; Gu, JR; Yang, SL; Gong, Y |
| 刊名 | Protein and peptide letters
 |
| 出版日期 | 2006 |
| 卷号 | 13期号:1页码:41-45 |
| 关键词 | Tumor suppressor gene Pp5715 Expression and purification Cell growth inhibition Subcellular localization |
| ISSN号 | 0929-8665 |
| 通讯作者 | Gong, y(yigong@srcb.ac.cn) |
| 英文摘要 | Pp5715 is a putative tumor suppressor gene that encodes a protein of 199 amino acids. expression of pp5715 in e.coli was mainly as inclusion bodies. the recombinant protein was purified by a nta-ni2+ affinity column, refolded by dialysis, and shown to suppress growth of two human hepatocellular carcinoma cell lines using the mtt assay or the clonogenic assays. computer analysis and fusion expression of pp5175 and gfp showed that pp5715 may be a secretory protein. it may bind to receptors on the membrane surface and thus induce regulation of cell growth. these preliminary results suggest that protein pp5715 may be a new tumor suppressor with growth inhibition effects on hepatocellular carcinoma cells. |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000233942400007 |
| 出版者 | BENTHAM SCIENCE PUBL LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378822 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Gong, Y |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Res Ctr Biotechnol, Grad Sch, Shanghai 200233, Peoples R China 2.Shanghai Jiao Tong Univ, Inst Canc, Natl Lab Oncogenes & Related Genes, Shanghai 200032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, WZ,Han, W,Qin, WX,et al. Expression and purification of a putative tumor suppressor gene pp5715 in e-coli with growth inhibition of hepatocellular carcinoma cells[J]. Protein and peptide letters,2006,13(1):41-45. |
| APA | Ma, WZ.,Han, W.,Qin, WX.,Wan, DF.,Gu, JR.,...&Gong, Y.(2006).Expression and purification of a putative tumor suppressor gene pp5715 in e-coli with growth inhibition of hepatocellular carcinoma cells.Protein and peptide letters,13(1),41-45. |
| MLA | Ma, WZ,et al."Expression and purification of a putative tumor suppressor gene pp5715 in e-coli with growth inhibition of hepatocellular carcinoma cells".Protein and peptide letters 13.1(2006):41-45. |
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来源：中国科学院大学
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