Mitochondrial reactive oxygen species and nitric oxide-mediated cancer cell apoptosis in 2-butylamino-2-demethoxyhypocrellin b photodynamic treatment
文献类型：期刊论文
| 作者 | Lu, Zhongbing; Tao, Yi; Zhou, Zhixiang; Zhang, Junjing; Li, Cong; Ou, Lingcheng; Zhao, Baolu |
| 刊名 | Free radical biology and medicine
 |
| 出版日期 | 2006-11-15 |
| 卷号 | 41期号:10页码:1590-1605 |
| 关键词 | Reactive oxygen species Nitric oxide Mitochondrial nitric oxide synthase Photodynamic therapy Cytochrome c |
| ISSN号 | 0891-5849 |
| DOI | 10.1016/j.freeradbiomed.2006.08.021 |
| 通讯作者 | Zhao, baolu(zhaobl@sun5.ibp.ac.cn) |
| 英文摘要 | Photodynamic therapy (pdt) is a novel and promising cancer treatment which employs a combination of a photosensitizing chemical and visible light to induce apoptosis in cancer cells. singlet oxygen has been recognized as the main origin of oxidative stress in pdt. however, the precise mechanism of pdt-induced apoptosis is not well characterized, especially the dualistic role of nitric oxide (no). to dissect the apoptosis pathways triggered by pdt, the intracellular free radicals in mcf-7 cells were investigated by examining a novel photosensitizer 2-butylamino-2-demethoxyhypocrellin b (2-ba-2-dmhb)-mediated pdt. it was found that exposure of the cells to 2-ba-2-dmhb and irradiation resulted in a significant increase of intracellular ros in minutes, and then followed by cytoplasmic free calcium enhancement, mitochondrial nitric oxide synthase (mtnos) activation, cytochrome c release, and apoptotic death. scavengers of singlet oxygen or no could attenuate pdt-induced cell viability loss, nucleus morphology changes, cytochrome c release, mitochondria swelling, and apo-apoptosis gene p53 and p21 mrna levels. the results suggested that both ros and no played important roles in the apoptosis-induced by pdt. (c) 2006 elsevier inc. all rights reserved. |
| WOS关键词 | CYTOPLASMIC FREE CALCIUM ; CYTOCHROME-C RELEASE ; HYDROGEN-PEROXIDE ; 5-AMINOLEVULINIC ACID ; SYNTHASE ACTIVITY ; FREE-RADICALS ; TUMOR-CELLS ; HELA-CELLS ; RAT-BRAIN ; THERAPY |
| WOS研究方向 | Biochemistry & Molecular Biology ; Endocrinology & Metabolism |
| WOS类目 | Biochemistry & Molecular Biology ; Endocrinology & Metabolism |
| 语种 | 英语 |
| WOS记录号 | WOS:000241563000009 |
| 出版者 | ELSEVIER SCIENCE INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378835 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhao, Baolu |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, State Key Lab Brain & Recognit Lab, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100029, Peoples R China 3.Univ Yuannan, Dept Chem, Kunming, Yunnan, Peoples R China 4.Shanghai Univ Trad Chinese Med, E Inst Shanghai Univ, Div Nitr Oxide & Inflammatory Med, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Zhongbing,Tao, Yi,Zhou, Zhixiang,et al. Mitochondrial reactive oxygen species and nitric oxide-mediated cancer cell apoptosis in 2-butylamino-2-demethoxyhypocrellin b photodynamic treatment[J]. Free radical biology and medicine,2006,41(10):1590-1605. |
| APA | Lu, Zhongbing.,Tao, Yi.,Zhou, Zhixiang.,Zhang, Junjing.,Li, Cong.,...&Zhao, Baolu.(2006).Mitochondrial reactive oxygen species and nitric oxide-mediated cancer cell apoptosis in 2-butylamino-2-demethoxyhypocrellin b photodynamic treatment.Free radical biology and medicine,41(10),1590-1605. |
| MLA | Lu, Zhongbing,et al."Mitochondrial reactive oxygen species and nitric oxide-mediated cancer cell apoptosis in 2-butylamino-2-demethoxyhypocrellin b photodynamic treatment".Free radical biology and medicine 41.10(2006):1590-1605. |
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来源：中国科学院大学
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