Expression, purification and crystallization of urokinase catalytic domain in pichia pastoris
文献类型：期刊论文
| 作者 | Zhao, GX; Yuan, C; Bian, CB; Jiang, LG; Ye, XM; Huang, ZX; Huang, MD |
| 刊名 | Progress in biochemistry and biophysics
 |
| 出版日期 | 2006-04-01 |
| 卷号 | 33期号:4页码:377-381 |
| 关键词 | Urokinase Pichia pastoris Plasminogen Site-mutated pcr Crystallization |
| ISSN号 | 1000-3282 |
| 通讯作者 | Huang, md(mhuang@fjirsm.ac.cn) |
| 英文摘要 | The gene fragment of urokinase catalytic domain mutant (c279a/n302q) was amplified by the site-mutated pcr method and was cloned into ppicz alpha a secretory expression plasmid. the recombinant plasmid was transformed into yeast x-33 and selected with zeocin. the recombinant protein was captured by the cation exchange chromatography spff and was purified to 99% of purity. the recombinant mutant protein was crystallized by the method of sitting-drop vapor diffusion. these crystals diffracted to 1.45 angstrom with synchrotron x-ray. |
| WOS关键词 | PLASMINOGEN-ACTIVATOR ; CRYSTAL-STRUCTURE ; CANCER CELLS ; INHIBITORS ; RECEPTOR ; PROTEASES ; OVARIAN ; SYSTEM |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000237049800011 |
| 出版者 | SCIENCE PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378907 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Huang, MD |
| 作者单位 | Grad Univ, Chinese Acad Sci, Fujian Inst Res Struct Matter, State Key Lab Struct Chem, Fuzhou 350002, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, GX,Yuan, C,Bian, CB,et al. Expression, purification and crystallization of urokinase catalytic domain in pichia pastoris[J]. Progress in biochemistry and biophysics,2006,33(4):377-381. |
| APA | Zhao, GX.,Yuan, C.,Bian, CB.,Jiang, LG.,Ye, XM.,...&Huang, MD.(2006).Expression, purification and crystallization of urokinase catalytic domain in pichia pastoris.Progress in biochemistry and biophysics,33(4),377-381. |
| MLA | Zhao, GX,et al."Expression, purification and crystallization of urokinase catalytic domain in pichia pastoris".Progress in biochemistry and biophysics 33.4(2006):377-381. |
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来源：中国科学院大学
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