Guanosine 5'-[gamma-thio]triphosphate-mediated activation of cytosol phospholipase c caused lysosomal destabilization
文献类型：期刊论文
| 作者 | Wang, Xiang; Wang, Li-Li; Zhang, Guo-Jiang |
| 刊名 | Journal of membrane biology
 |
| 出版日期 | 2006-05-01 |
| 卷号 | 211期号:1页码:55-63 |
| 关键词 | Lysosome Gtp-gamma-s Phospholipase c Ion permeability Osmotic sensitivity |
| ISSN号 | 0022-2631 |
| DOI | 10.1007/s00232-006-0019-0 |
| 通讯作者 | Zhang, guo-jiang(zhanggj@sun5.ibp.ac.cn) |
| 英文摘要 | Lysosomal disintegration is critical for the organelle functions and cellular viability. in this study, we established that guanosine 5'-[gamma-thio]triphosphate (gtp-gamma-s)-activated cytosol of rat hepatocytes could increase lysosomal permeability to both potassium ions and protons and osmotically destabilize the lysosomes via k+/h+ exchange. these results were obtained through measurements of lysosomal beta-hexosaminidase-free activity, membrane potential and intralysosomal ph. assays of phospholipase c (plc) activity show that cytosolic plc was activated upon addition of gtp-gamma-s to the cytosol. the effects of cytosol on the lysosomes could be abolished by d609, an inhibitor of plc, but not by the inhibitors of phospholipase a(2). the cytosol-treated lysosomes disintegrated markedly in hypotonic sucrose medium, reflecting that the lysosomal osmotic sensitivity increased. microscopic observations showed that the lysosomes became more swollen in hypotonic sucrose medium. this indicates that the cytosol treatment induced osmotic shock to the lysosomes and an influx of water into the organelle. |
| WOS关键词 | CARDIAC MYOCYTES ; PROTON PUMP ; RAT-LIVER ; MEMBRANE ; APOPTOSIS ; PERMEABILITY ; TRANSPORT ; CALCIUM ; CELLS ; INVOLVEMENT |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology ; Physiology |
| WOS类目 | Biochemistry & Molecular Biology ; Cell Biology ; Physiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000240853800006 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378959 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhang, Guo-Jiang |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiang,Wang, Li-Li,Zhang, Guo-Jiang. Guanosine 5'-[gamma-thio]triphosphate-mediated activation of cytosol phospholipase c caused lysosomal destabilization[J]. Journal of membrane biology,2006,211(1):55-63. |
| APA | Wang, Xiang,Wang, Li-Li,&Zhang, Guo-Jiang.(2006).Guanosine 5'-[gamma-thio]triphosphate-mediated activation of cytosol phospholipase c caused lysosomal destabilization.Journal of membrane biology,211(1),55-63. |
| MLA | Wang, Xiang,et al."Guanosine 5'-[gamma-thio]triphosphate-mediated activation of cytosol phospholipase c caused lysosomal destabilization".Journal of membrane biology 211.1(2006):55-63. |
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来源：中国科学院大学
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