Identification and characterization of a novel heat shock transcription factor gene, gmhsfa1, in soybeans (glycine max)
文献类型：期刊论文
| 作者 | Zhu, Baoge; Ye, Chunjiang; L, Huiying; Chen, Xiaojun; Chai, Guohua; Chen, Jiannan; Wang, Chao |
| 刊名 | Journal of plant research
 |
| 出版日期 | 2006-05-01 |
| 卷号 | 119期号:3页码:247-256 |
| 关键词 | Glycine max Heat shock transcription factor gene Transformation Overexpression Thermotolerance Heat stress |
| ISSN号 | 0918-9440 |
| DOI | 10.1007/s10265-006-0267-1 |
| 通讯作者 | Zhu, baoge(bgzhu@genetics.ac.cn) |
| 英文摘要 | Plants have a large family of hsfs with different roles in the heat shock response that mediate the expression of hsp regulated genes. the hsf encoding genes are easily identified by their highly conserved modular structure and motifs. in the present study, a putative gmhsfa1 was identified and characterized from the soybean expressed sequence tag (est) database by sequence comparison with the functionally well-characterized lphsfa1 and rapid amplification of cdna ends (race). multiple alignment showed that the amino acid sequence of gmhsfa1, matching best with lphsfa1 (52.2% similarity), was obviously different from that of each of several hsfa1s from other plant species. the gmhsfa1 has a constitutive expression profile in the different tissues examined. the overexpression of gmhsfa1 in transgenic soybean plants led to the activation of gmhsp70 under normal temperature and the overexpression of gmhsp70 under high temperature. furthermore, transgenic soybean plants with gmhsfa1 overexpression showed obvious enhancement of thermotolerance under heat stress in comparison with non-transgenic plants. the experimental results suggested that gmhsfa1 is a novel and functional heat-shock transcription factor. |
| WOS关键词 | COTYLEDONARY-NODE CELLS ; NUCLEAR IMPORT ; PROTEINS ; TOMATO ; HSFA1 ; THERMOTOLERANCE ; TRIMERIZATION ; ARABIDOPSIS ; ACTIVATION ; DOMAINS |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000238004300009 |
| 出版者 | SPRINGER TOKYO |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2378993 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhu, Baoge |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Xinjiang Agr Univ, Xining 830052, Peoples R China 4.NW Sci Tech Univ Agr & Forestry, Shanxi 712100, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Baoge,Ye, Chunjiang,L, Huiying,et al. Identification and characterization of a novel heat shock transcription factor gene, gmhsfa1, in soybeans (glycine max)[J]. Journal of plant research,2006,119(3):247-256. |
| APA | Zhu, Baoge.,Ye, Chunjiang.,L, Huiying.,Chen, Xiaojun.,Chai, Guohua.,...&Wang, Chao.(2006).Identification and characterization of a novel heat shock transcription factor gene, gmhsfa1, in soybeans (glycine max).Journal of plant research,119(3),247-256. |
| MLA | Zhu, Baoge,et al."Identification and characterization of a novel heat shock transcription factor gene, gmhsfa1, in soybeans (glycine max)".Journal of plant research 119.3(2006):247-256. |
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来源：中国科学院大学
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