A proteomic investigation into a human gastric cancer cell line bgc823 treated with diallyl trisulfide
文献类型：期刊论文
| 作者 | Li, Na; Guo, Ruifang; Li, Wenmei; Shao, Jianmin; Li, Shuting; Zhao, Kang; Chen, Xishu; Xu, Ningzhi; Liu, Siqi; Lu, Youyong |
| 刊名 | Carcinogenesis
 |
| 出版日期 | 2006-06-01 |
| 卷号 | 27期号:6页码:1222-1231 |
| ISSN号 | 0143-3334 |
| DOI | 10.1093/carcin/bgi306 |
| 通讯作者 | Lu, youyong(yongylu@public.bta.net.cn) |
| 英文摘要 | Garlic is generally used as a therapeutic reagent against various diseases worldwide. although a great effort is made to understand the pharmaceutical mechanisms of garlic and its derivatives, there are many mysteries to be uncovered. using proteomic means, herein we have systematically studied the responses of protein expression in bgc823 cells, a gastric cancer cell line, induced by diallyl trisulfide (dats), a major component of garlic derivatives. a total of 41 unique proteins in bgc823 were detected with significant changes in their expression levels corresponding with dats administration. of these proteins, five typical ones, glutathione s-transferase-pi (gst-pi), voltage-dependent anion channel-1 (vdac-1), annexin 1, galectin and s100a11, were further examined by western blotting, resulting in coincident data with the proteomic evidence. moreover quantitative real-time rt-pcr experiments offered dynamic data of mrna expression, indicating the responses of annexin i and gst-pi expression within a short period after dats treatment. interestingly, similar to 50% of dats-sensitive proteins (19/41) in bgc823 are tightly associated with apoptotic pathways. these proteomic results presented, therefore, provide additional support to the hypothesis that garlic is a strong inducer of apoptosis in tumor cells. |
| WOS关键词 | BCL-2 FAMILY PROTEINS ; N-TERMINAL KINASE ; APOPTOSIS INDUCTION ; EPITHELIAL-CELLS ; CYTOCHROME-C ; CYCLE ARREST ; EXPRESSION ; DISULFIDE ; RELEASE ; DEATH |
| WOS研究方向 | Oncology |
| WOS类目 | Oncology |
| 语种 | 英语 |
| 出版者 | OXFORD UNIV PRESS |
| WOS记录号 | WOS:000238816700014 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379008 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Lu, Youyong |
| 作者单位 | 1.Chinese Acad Sci, Beijing Genom Inst, Beijing 101300, Peoples R China 2.Beijing Proteom Inst, Beijing 101300, Peoples R China 3.Peking Univ, Sch Oncol, Beijing Inst Canc Res, Beijing 100034, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Na,Guo, Ruifang,Li, Wenmei,et al. A proteomic investigation into a human gastric cancer cell line bgc823 treated with diallyl trisulfide[J]. Carcinogenesis,2006,27(6):1222-1231. |
| APA | Li, Na.,Guo, Ruifang.,Li, Wenmei.,Shao, Jianmin.,Li, Shuting.,...&Lu, Youyong.(2006).A proteomic investigation into a human gastric cancer cell line bgc823 treated with diallyl trisulfide.Carcinogenesis,27(6),1222-1231. |
| MLA | Li, Na,et al."A proteomic investigation into a human gastric cancer cell line bgc823 treated with diallyl trisulfide".Carcinogenesis 27.6(2006):1222-1231. |
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来源：中国科学院大学
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