Dedifferentiation of adult monkey sertoli cells through activation of extracellularly regulated kinase 1/2 induced by heat treatment
文献类型：期刊论文
| 作者 | Zhang, XS; Zhang, ZH; Jin, X; Wei, P; Hu, XQ; Chen, M; Lu, CL; Lue, YH; Hu, ZY; Hikim, APS |
| 刊名 | Endocrinology
 |
| 出版日期 | 2006-03-01 |
| 卷号 | 147期号:3页码:1237-1245 |
| ISSN号 | 0013-7227 |
| DOI | 10.1210/en.2005-0981 |
| 通讯作者 | Liu, yx() |
| 英文摘要 | Sertoli cells play a key role in triggering and regulating the process of spermatogenesis. failure of a sertoli cell to mature functionally will presumably render it incapable of supporting germ cell survival and development that appeared after puberty. expression of cytokeratin 18 (ck-18) intermediate filaments indicates a state of undifferentiation usually observed in sertoli cells of prepubertal testis. in this study we demonstrated that local testicular heat treatment of adult monkey with water at 43 c for 30 min once daily for 2 consecutive days was capable of activating reexpression of ck-18 in sertoli cells, which was coincident with activation of erk1/2 and akt kinases. using primary sertoli cell culture isolated from adult monkey testis, we further confirmed that the heat treatment of the cells at 43 c could also induce ck-18 reexpression, which was similar to the in vivo treatment. erk mapk was also induced by the heat treatment in a time- and protein kinase a (pka)- dependent manner. after blocking the erk mapk signaling pathway, an inhibition of ck-18 expression in the cultured sertoli cells was observed, and this inhibitory effect was also detected by blocking the pka activation. however, ck-18 activation in sertoli cells remained unaltered when the phosphatidylinositol 3-kinase/akt pathway was blocked. in conclusion, the heat treatment of adult monkey sertoli cells are capable of inducing a reversible change in the sertoli cells from an adult differentiated state to an immature-like dedifferentiated state through pka-erk mapk-dependent pathways but not via the phosphatidylinositol 3-kinase/ akt pathway. |
| WOS关键词 | PROTEIN-KINASE ; EXPERIMENTAL CRYPTORCHIDISM ; MACACA-FASCICULARIS ; RAT SERTOLI ; PHOSPHATIDYLINOSITOL 3-KINASE ; INTERMEDIATE-FILAMENTS ; SIGNAL-TRANSDUCTION ; TESTICULAR TISSUE ; HUMAN TESTIS ; EXPRESSION |
| WOS研究方向 | Endocrinology & Metabolism |
| WOS类目 | Endocrinology & Metabolism |
| 语种 | 英语 |
| WOS记录号 | WOS:000235350100023 |
| 出版者 | ENDOCRINE SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379009 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Liu, YX |
| 作者单位 | 1.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100080, Peoples R China 2.Harbor UCLA Med Ctr, Dept Med, Div Endocrinol, Torrance, CA 90509 USA 3.Los Angeles Biomed Res inst, Torrance, CA 90509 USA 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, XS,Zhang, ZH,Jin, X,et al. Dedifferentiation of adult monkey sertoli cells through activation of extracellularly regulated kinase 1/2 induced by heat treatment[J]. Endocrinology,2006,147(3):1237-1245. |
| APA | Zhang, XS.,Zhang, ZH.,Jin, X.,Wei, P.,Hu, XQ.,...&Liu, YX.(2006).Dedifferentiation of adult monkey sertoli cells through activation of extracellularly regulated kinase 1/2 induced by heat treatment.Endocrinology,147(3),1237-1245. |
| MLA | Zhang, XS,et al."Dedifferentiation of adult monkey sertoli cells through activation of extracellularly regulated kinase 1/2 induced by heat treatment".Endocrinology 147.3(2006):1237-1245. |
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来源：中国科学院大学
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