Identification of atent3 as the main transporter for uridine uptake in arabidopsis roots
文献类型：期刊论文
| 作者 | Chen, KL; Xu, MX; Li, GY; Liang, H; Xia, ZL; Liu, X; Zhang, JS; Zhang, AM; Wang, DW |
| 刊名 | Cell research
 |
| 出版日期 | 2006-04-01 |
| 卷号 | 16期号:4页码:377-388 |
| 关键词 | Arabidopsis Equilibrative nucleoside transporter Expression pattern Nucleoside transport Functional analysis |
| ISSN号 | 1001-0602 |
| DOI | 10.1038/sj.cr.7310049 |
| 通讯作者 | Wang, dw() |
| 英文摘要 | Previous studies have shown that arabidopsis equilibrative nucleoside transporters (atents) possess transport activities when produced in yeast cells and are differentially expressed in arabidopsis organs. herein, we report further analysis on the nucleoside transport activities and transcriptional patterns of atent members. the recombinant proteins of atents 3, 6, and 7, but not those of atents 1, 2, 4, and 8, were found to transport thymidine with high affinity. contrary to previous suggestion that atent 1 may not transport uridine, this work showed that recombinant atent 1 was a ph-dependent and high-affinity transporter of uridine. when grown on ms plates, the atent3 knockout plants were more tolerant to the cytotoxic uridine analog 5-fluorouridine than wild-type plants and the knockout plants of atent 1 or atent6. consistent with this observation, the atent3 knockout line exhibited a significantly decreased ability to take up [h-3]uridine via the roots when compared with wild-type plants and the plants with mutated atent 1 or atent6. this indicates that atent3, but not atents 1 and 6, is the main transporter for uridine uptake in arabidopsis roots. the transcription of atents 1, 3, 4, 6, 7, and 8, was regulated in a complex manner during leaf development and senescence. in contrast, the six atent members were coordinately induced during seed germination. this work provides new information on the transport properties of recombinant atent proteins and new clues for future studies of the in vivo transport activities and physiological functions of the different ent proteins in arabidopsis plants. |
| WOS关键词 | EQUILIBRATIVE NUCLEOSIDE TRANSPORTER ; SPRUCE PICEA-GLAUCA ; SACCHAROMYCES-CEREVISIAE ; INTRACELLULAR MEMBRANES ; FUNCTIONAL-CHARACTERIZATION ; PYRIMIDINE NUCLEOTIDE ; CYTOKININ TRANSPORT ; MOLECULAR-BIOLOGY ; THYMIDINE KINASE ; MAMMALIAN-CELLS |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000237837400006 |
| 出版者 | INST BIOCHEMISTRY & CELL BIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379033 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, DW |
| 作者单位 | 1.Chinese Acad Sci, Ctr Plant Gene Res Beijing, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China 3.NW Agr & Forestry Univ, Sch Life Sci, Yangling 712100, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, KL,Xu, MX,Li, GY,et al. Identification of atent3 as the main transporter for uridine uptake in arabidopsis roots[J]. Cell research,2006,16(4):377-388. |
| APA | Chen, KL.,Xu, MX.,Li, GY.,Liang, H.,Xia, ZL.,...&Wang, DW.(2006).Identification of atent3 as the main transporter for uridine uptake in arabidopsis roots.Cell research,16(4),377-388. |
| MLA | Chen, KL,et al."Identification of atent3 as the main transporter for uridine uptake in arabidopsis roots".Cell research 16.4(2006):377-388. |
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来源：中国科学院大学
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