Infective viruses produced from full-length complementary dna of swine vesicular disease viruses hk/70 strain
文献类型：期刊论文
| 作者 | Zheng Haixue; Liu Xinagtao; Shang Youjun; Wu Jinyan; Bai Xingwen; Jin Ye; Sun Shiqi; Guo Huichen; Tian Hong; Feng Xia |
| 刊名 | Chinese science bulletin
 |
| 出版日期 | 2006-09-01 |
| 卷号 | 51期号:17页码:2072-2078 |
| 关键词 | Swine vesicular disease virus Full-length cdna Infectious clone Psvok(12) Reverse genetics |
| ISSN号 | 1001-6538 |
| DOI | 10.1007/s11434-006-2095-z |
| 通讯作者 | Liu xinagtao(xieqgkey@public.lz.gs.cn) |
| 英文摘要 | The full-length cdna clone of swine vesicular disease virus hk/70 strain named psvok(12) was constructed in order to study the antigenicity, replication, maturation and pathogenicity of swine vesicular disease virus. in vitro transcription rna from psvok(12) transfected ibrs-2 cells and the recovered virus rna were isolated and sequenced, then indirect hemagglutination test, indirect immunofluorescence assays, eleectron microscope test, 50% tissue culture infecting dose (tcid50) assays and mouse virulence studies were performed to study the antigenicity and virulence of the recovered virus. the result showed that the infectious clones we obtained and the virus derived from psvok(12) had the same biological properties as the parental strain hk/70. the full-length infectious cdna clone, psvok(12), will be very useful in studies of the antigenicity, virulence, pathogenesis, maturation and replication of svdv. |
| WOS关键词 | COMPLETE NUCLEOTIDE-SEQUENCE ; COXSACKIE B5 VIRUS ; MAMMALIAN-CELLS ; CDNA ; RNA ; FOOT ; ATTENUATION ; TRANSCRIPTS ; VARIANTS ; SEGMENT |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000241849200007 |
| 出版者 | SCIENCE CHINA PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379079 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Liu Xinagtao |
| 作者单位 | 1.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Minist Agr,Key Lab Anim Virol, Lanzhou 730046, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100081, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng Haixue,Liu Xinagtao,Shang Youjun,et al. Infective viruses produced from full-length complementary dna of swine vesicular disease viruses hk/70 strain[J]. Chinese science bulletin,2006,51(17):2072-2078. |
| APA | Zheng Haixue.,Liu Xinagtao.,Shang Youjun.,Wu Jinyan.,Bai Xingwen.,...&Xie Qingge.(2006).Infective viruses produced from full-length complementary dna of swine vesicular disease viruses hk/70 strain.Chinese science bulletin,51(17),2072-2078. |
| MLA | Zheng Haixue,et al."Infective viruses produced from full-length complementary dna of swine vesicular disease viruses hk/70 strain".Chinese science bulletin 51.17(2006):2072-2078. |
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来源：中国科学院大学
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