Sub-attomole oligonucleotide and p53 cdna determinations via a high-resolution surface plasmon resonance combined with oligonucleotide-capped gold nanoparticle signal amplification
文献类型：期刊论文
| 作者 | Yao, Xin; Li, Xin; Toledo, Freddy; Zurita-Lopez, Cecilia; Gutova, Margarita; Momand, Jamil; Zhou, Feimeng |
| 刊名 | Analytical biochemistry
 |
| 出版日期 | 2006-07-15 |
| 卷号 | 354期号:2页码:220-228 |
| 关键词 | Dna analysis Surface plasmon resonance Differential signal detection Dna-capped gold nanoparticles Carboxylated dextran |
| ISSN号 | 0003-2697 |
| DOI | 10.1016/j.ab.2006.04.011 |
| 通讯作者 | Zhou, feimeng(fzhou@calstatela.edu) |
| 英文摘要 | Oligonucleotide (odn)-capped gold nanoparticles (au-nps) were used in a sandwich assay of odn or polynucleotide by a flow injection surface plasmon resonance (spr). a carboxylated dextran film was immobilized onto the spr sensor surface to eliminate nonspecific adsorption of odn-capped au-nps. the tandem use of signal amplification via the adlayer of the odn-capped au-nps and the differential signal detection by the bicell detector on the spr resulted in a remarkable dna detection level. a 39-mer target at a quantity as low as 2.1 x 10(-20) mol, corresponding to 1.38 fm in a 15 mu l solution, can be measured. to our knowledge, both the concentration and quantity detection levels are the lowest among all the gene analyses conducted with spr to this point. the method is shown to be reproducible (relative standard deviation values < 16%) and to possess high sequence specificity. it is also demonstrated to be viable for sequence-specific p53 cdna analysis. the successful elimination of nonspecific adsorption of, and the signal amplification by, odn-capped au-nps renders the spr attractive for cases where the dna concentration is extremely low and the sample availability is severely limited. (c) 2006 elsevier inc. all rights reserved. |
| WOS关键词 | POOR-PROGNOSIS ; DNA HYBRIDIZATION ; ULTRASENSITIVE DETECTION ; IMAGING MEASUREMENTS ; OPTICAL BIOSENSOR ; RNA MICROARRAYS ; GENE-EXPRESSION ; QUARTZ-CRYSTAL ; NUCLEIC-ACIDS ; BINDING |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000238890500008 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379081 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhou, Feimeng |
| 作者单位 | 1.Calif State Univ Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90032 USA 2.Chinese Acad Sci, Grad Sch, Coll Chem & Chem Engn, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yao, Xin,Li, Xin,Toledo, Freddy,et al. Sub-attomole oligonucleotide and p53 cdna determinations via a high-resolution surface plasmon resonance combined with oligonucleotide-capped gold nanoparticle signal amplification[J]. Analytical biochemistry,2006,354(2):220-228. |
| APA | Yao, Xin.,Li, Xin.,Toledo, Freddy.,Zurita-Lopez, Cecilia.,Gutova, Margarita.,...&Zhou, Feimeng.(2006).Sub-attomole oligonucleotide and p53 cdna determinations via a high-resolution surface plasmon resonance combined with oligonucleotide-capped gold nanoparticle signal amplification.Analytical biochemistry,354(2),220-228. |
| MLA | Yao, Xin,et al."Sub-attomole oligonucleotide and p53 cdna determinations via a high-resolution surface plasmon resonance combined with oligonucleotide-capped gold nanoparticle signal amplification".Analytical biochemistry 354.2(2006):220-228. |
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来源：中国科学院大学
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