Berberine inhibits 3t3-l1 adipocyte differentiation through the ppar gamma pathway
文献类型：期刊论文
| 作者 | Huang, Cheng; Zhang, Yuebo; Gong, Zhenwei; Sheng, Xiaoyan; Li, Zongmeng; Zhang, Wei; Qin, Ying |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2006-09-22 |
| 卷号 | 348期号:2页码:571-578 |
| 关键词 | Berberine 3t3-l1 Ppar gamma Adipocyte differentiation Obesity Diabetes |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2006.07.095 |
| 通讯作者 | Qin, ying(yqin@sibs.ac.cn) |
| 英文摘要 | Berberine (bbr), a compound purified from cortidis rhizoma, reduces serum cholesterol, triglycerides, and ldl-cholesterol of hyper-cholesterolemic patients and high fat diet fed animals, and increases hepatic ldlr mrna and protein levels through a post-transcriptional mechanism. bbr also enhances the hypoglycemic action of insulin in diabetic animal models. here, we show that bbr inhibits the differentiation of 3t3-l1 preadipocytes induced by dm and suppresses the mitotic clonal expansion of 3t3-l1 preadipocytes in a time-and dose-dependent manner. gene expression analysis and western blot analysis reveal that the bbr inhibits the mrna and protein levels of adipogenesis related transcription factors ppar gamma and c/ebp alpha and their upstream regulator, c/ebp beta. reporter gene assays demonstrate that the full-length ppar gamma and alpha transcription activities are inhibited by bbr. using real-time pcr, we have also found that the ppar target genes that are involved in adipocyte differentiation, such as ap2, cd36, aco, lpl, and other adipocyte markers, are suppressed by bbr. these studies suggest that bbr works on multiple molecular targets as an inhibitor of ppar gamma and alpha, and is a potential weight reducing, hypolipidemic, and hypoglycemic drug. (c) 2006 elsevier inc. all rights reserved. |
| WOS关键词 | MITOTIC CLONAL EXPANSION ; ACTIVATED RECEPTOR-GAMMA ; C/EBP-BETA ; ADIPOGENESIS ; EXPRESSION ; MECHANISM ; CELLS ; TRANSCRIPTION ; FIBROBLASTS ; AGONISTS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000240275000033 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379090 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Qin, Ying |
| 作者单位 | Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Inst Nutr Sci, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Cheng,Zhang, Yuebo,Gong, Zhenwei,et al. Berberine inhibits 3t3-l1 adipocyte differentiation through the ppar gamma pathway[J]. Biochemical and biophysical research communications,2006,348(2):571-578. |
| APA | Huang, Cheng.,Zhang, Yuebo.,Gong, Zhenwei.,Sheng, Xiaoyan.,Li, Zongmeng.,...&Qin, Ying.(2006).Berberine inhibits 3t3-l1 adipocyte differentiation through the ppar gamma pathway.Biochemical and biophysical research communications,348(2),571-578. |
| MLA | Huang, Cheng,et al."Berberine inhibits 3t3-l1 adipocyte differentiation through the ppar gamma pathway".Biochemical and biophysical research communications 348.2(2006):571-578. |
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来源：中国科学院大学
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