Cloning of a cdna encoding the saussurea medusa chalcone isornerase and its expression in transgenic tobacco
文献类型：期刊论文
| 作者 | Li, F.; Jin, Z.; Qu, W.; Zhao, D.; Ma, F. |
| 刊名 | Plant physiology and biochemistry
 |
| 出版日期 | 2006-07-01 |
| 卷号 | 44期号:7-9页码:455-461 |
| ISSN号 | 0981-9428 |
| 关键词 | Anthocyanin Chalcone isomerase Flavonoids Rutin Saussurea medusa Tobacco |
| DOI | 10.1016/j.plaphy.2006.08.006 |
| 通讯作者 | Zhao, d.(zhaodx@ibcas.ac.cn) |
| 英文摘要 | Chalcone isomerase (chi; ec 5.5.1.6) is a key enzyme in the flavonoid biosynthesis pathway. we isolated a chi gene (smchi) from a cdna library derived from saussurea medusa (asteraceae) cell cultures. the cdna and genomic sequences of smchi are the same; in other words, this gene is intronless. the coding region of the gene is 699 bp long, and its deduced protein consists of 232 amino acids with a predicted molecular mass of 24 kda and a pi of 4.7. the deduced amino acid sequence of smchi shares 79.3% identity with chi from callistephus chinensis, a familial relative to s. medusa; this homology is higher than those with chi's from any other plant species. a functional bioassay for smchi was performed by transforming nicotiana tabacum plants in the sense or antisense orientation under the regulation of the cauliflower mosaic virus (camv) 35s promoter. transgenic tobacco plants overexpressing sense smchi produced up to fivefold total flavonoids over wildtype tobacco plants, mainly due to an enhanced accumulation of rutin. transgenic tobacco plants with antisense smchi accumulated smaller amounts of flavonoids; this is apparently brought about by suppressed expression of the endogenous chi gene. chi activities also positively correlated with the amounts of total flavonoids accumulated in the transgenic plants. it is concluded that overexpression of smchi can be used as a useful approach to increase flavonoid production in transgenic plants. (c) 2006 elsevier masson sas. all rights reserved. |
| WOS关键词 | FLAVANONE ISOMERASE ; FLAVONOIDS ; PLANT ; PURIFICATION ; RISK ; BIOSYNTHESIS ; ANTIOXIDANTS ; QUERCETIN ; MECHANISM ; ARTHRITIS |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| 出版者 | ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER |
| WOS记录号 | WOS:000241857800003 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379230 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhao, D. |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Univ Waterloo, Dept Biol, Waterloo, ON N2L 3G1, Canada |
| 推荐引用方式 GB/T 7714 | Li, F.,Jin, Z.,Qu, W.,et al. Cloning of a cdna encoding the saussurea medusa chalcone isornerase and its expression in transgenic tobacco[J]. Plant physiology and biochemistry,2006,44(7-9):455-461. |
| APA | Li, F.,Jin, Z.,Qu, W.,Zhao, D.,&Ma, F..(2006).Cloning of a cdna encoding the saussurea medusa chalcone isornerase and its expression in transgenic tobacco.Plant physiology and biochemistry,44(7-9),455-461. |
| MLA | Li, F.,et al."Cloning of a cdna encoding the saussurea medusa chalcone isornerase and its expression in transgenic tobacco".Plant physiology and biochemistry 44.7-9(2006):455-461. |
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来源：中国科学院大学
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