Molecular cloning and characterization of a glutathione s-transferase gene repressed by phenylacetic acid from penicillium chrysogenum
文献类型：期刊论文
| 作者 | Wang Fu-Qiang; Zheng Gui-Zhen; Zhao Ying; Ren Zhi-Hong; Jia Qian; He Jian-Gong; Yu Jun |
| 刊名 | Progress in biochemistry and biophysics
 |
| 出版日期 | 2006-12-01 |
| 卷号 | 33期号:12页码:1223-1230 |
| 关键词 | Penicillium chrysogenum Glutathione s-transferase Phenylacetic acid |
| ISSN号 | 1000-3282 |
| 通讯作者 | Yu jun(junyu@genomics.org.cn) |
| 英文摘要 | Glutathione s-transferase (gst) gene, pcgsta was cloned from the penicillin producing strain penicillium chrysogenum, which is important for understanding the industrial fermentation process. pcgsta gene has an open-reading-frame of 840 bp in length, which is interrupted by two introns. the deduced amino acid sequence shows about 50% identity to several characterized filamentous fungi gsts. the recombinant pcgsta in escherichia coli were overexpressed and purified. enzymatic assays showed that the recombinant pcgsta had a specific activity with 1-chloro-2, 4-dinitrobenzene of (0.159 +/- 0.031) mu mol/(min(.)mg). it was found that the expression level of pcgsta in the penicillin producing medium supplemented with phenylacetic acid, the side chain precursor of penicillin g, was significant down regulated than that in medium without phenylacetic acid. this result suggested that pcgst may be related to phenylacetic acid metabolism in the penicillin producing strain. |
| WOS关键词 | ISOPENICILLIN-N SYNTHETASE ; FILAMENTOUS FUNGI ; DEPENDENT DETOXIFICATION ; BIOSYNTHESIS ; EXPRESSION ; ENZYME ; RESISTANCE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000243097200011 |
| 出版者 | CHINESE ACAD SCIENCES, INST BIOPHYSICS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379257 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yu Jun |
| 作者单位 | 1.Chinese Acad Sci, Beijing Genom Inst, Beijing 101300, Peoples R China 2.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 4.N China Pharmaceut Corp, New Drug R&D Ctr, Shijiazhuang 050015, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang Fu-Qiang,Zheng Gui-Zhen,Zhao Ying,et al. Molecular cloning and characterization of a glutathione s-transferase gene repressed by phenylacetic acid from penicillium chrysogenum[J]. Progress in biochemistry and biophysics,2006,33(12):1223-1230. |
| APA | Wang Fu-Qiang.,Zheng Gui-Zhen.,Zhao Ying.,Ren Zhi-Hong.,Jia Qian.,...&Yu Jun.(2006).Molecular cloning and characterization of a glutathione s-transferase gene repressed by phenylacetic acid from penicillium chrysogenum.Progress in biochemistry and biophysics,33(12),1223-1230. |
| MLA | Wang Fu-Qiang,et al."Molecular cloning and characterization of a glutathione s-transferase gene repressed by phenylacetic acid from penicillium chrysogenum".Progress in biochemistry and biophysics 33.12(2006):1223-1230. |
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来源：中国科学院大学
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