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Chinese Academy of Sciences Institutional Repositories Grid
Petunia germinating pollen s/d3 interacts with s-rnases in petunia hybrida vilm.

文献类型:期刊论文

作者Guo, YX; Zhang, YS; Xue, YB
刊名Journal of integrative plant biology
出版日期2006-05-01
卷号48期号:5页码:584-590
关键词Petunia germinating pollen (pgp) s/d3 Petunia hybrida Self-incompatibility S-rnase
ISSN号1672-9072
通讯作者Xue, yb()
英文摘要Self-incompatibility (si) is a genetic mechanism of self/non-self pollen recognition to prevent self-fertilization in many flowering plants and, in most cases, this is controlled by a multi-allelic s-locus. s-rnase and s-locus f box (slf) proteins have been shown to be the female and male determinants of gametophytic self-incompatibility (gsi), respectively, in the solanaceae, scrophulariaceae and rosaceae. nevertheless, it is thought that additional factors are required for the si response. herein, we constructed a mature anther cdna library from a self-incompatible petunia hybrida vilm. line of the s3s3 haplotype. using ahs2-rnase from antirrhinum hispanicum as a bait for yeast two-hybrid screening, we found that petunia germinating pollen (pgp) s/d3 was capable of interacting physically with the bait. however, the interaction lacked haplotype specificity. the pgps/d3 gene is a single copy gene that is expressed in tissues such as the style, ovary, pollen, and leaf. the pgps/d3::gfp (green fluorescence protein) construct was detected in both the membrane and cytoplasm. the implications of these findings in the operation of s-rnase-based si are discussed.
WOS关键词GAMETOPHYTIC SELF-INCOMPATIBILITY ; CLATHRIN-MEDIATED ENDOCYTOSIS ; NICOTIANA-ALATA ; SOLANUM-CHACOENSE ; BINDING PROTEIN ; IDENTIFICATION ; REJECTION ; DYNAMIN ; TUBES ; GENE
WOS研究方向Biochemistry & Molecular Biology ; Plant Sciences
WOS类目Biochemistry & Molecular Biology ; Plant Sciences
语种英语
WOS记录号WOS:000237257600012
出版者BLACKWELL PUBLISHING
URI标识http://www.irgrid.ac.cn/handle/1471x/2379268
专题中国科学院大学
通讯作者Xue, YB
作者单位1.Chinese Acad Sci, Inst Genet & Dev Biol, Lab Mol & Dev Biol, Beijing 100080, Peoples R China
2.Natl Ctr Plant Gene Res, Beijing 100080, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
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GB/T 7714
Guo, YX,Zhang, YS,Xue, YB. Petunia germinating pollen s/d3 interacts with s-rnases in petunia hybrida vilm.[J]. Journal of integrative plant biology,2006,48(5):584-590.
APA Guo, YX,Zhang, YS,&Xue, YB.(2006).Petunia germinating pollen s/d3 interacts with s-rnases in petunia hybrida vilm..Journal of integrative plant biology,48(5),584-590.
MLA Guo, YX,et al."Petunia germinating pollen s/d3 interacts with s-rnases in petunia hybrida vilm.".Journal of integrative plant biology 48.5(2006):584-590.

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来源:中国科学院大学

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