Critical role of ask1 in the 6-hydroxydopamine-induced apoptosis in human neuroblastoma sh-sy5y cells
文献类型：期刊论文
| 作者 | Ouyang, MX; Shen, X |
| 刊名 | Journal of neurochemistry
 |
| 出版日期 | 2006-04-01 |
| 卷号 | 97期号:1页码:234-244 |
| 关键词 | Apoptosis Apoptosis signal-regulating kinase 1 C-jun n-terminal kinase 6-hydroxydopamine P38 mitogen-activated protein kinase Parkinson's disease |
| ISSN号 | 0022-3042 |
| DOI | 10.1111/j.1471-4159.2006.03730.x |
| 通讯作者 | Shen, x() |
| 英文摘要 | 6-hydroxydopamine (6-ohda)-induced apoptosis in dopaminergic neuronal cells is a common cell model of parkinson's disease (pd). the role of apoptosis signal-regulating kinase 1 (ask1) in this model has not been well studied. we observed significant activation of ask1, p38 and jnk, as well as apoptosis in human dopaminergic neuroblastoma sh-sy5y cells exposed to 6-ohda. over-expressing kinase-dead mutant ask1(k709m) or knock-down of endogenous ask1 by its small interfering rna (sirna) greatly suppressed activation of these kinases and apoptosis in the cells. it was found that the activation of p38 and jnk was suppressed to almost the same extent as that of ask1 in the ask1-knock-down cells, suggesting that activated ask1 is almost totally responsible for activation of p38/jnk. it was also observed that the 6-ohda-induced cell apoptosis could be effectively prevented by over-expressing the dominant-negative mutant of p38 or p38 inhibitor sb203580, demonstrating that activation of p38/jnk signalling is required for initiating the programmed cell death. furthermore, suppression of the 6-ohda-generated reactive oxygen species (ros) by pre-incubation of cells with n-acetyl-l-cysteine effectively inhibited the 6-ohda-induced activation of ask1, p38 and jnk, and protected the cells from apoptosis. this study clearly shows the route from ros generation by 6-ohda to initiation of p38/jnk signalling via activation of ask1 in the studied pd model. |
| WOS关键词 | ACTIVATED PROTEIN-KINASE ; PARKINSONS-DISEASE ; OXIDATIVE STRESS ; HYDROGEN-PEROXIDE ; DOPAMINERGIC-NEURONS ; SUBSTANTIA-NIGRA ; ANIMAL-MODELS ; CYTOCHROME-C ; PC12 CELLS ; P38 MAPK |
| WOS研究方向 | Biochemistry & Molecular Biology ; Neurosciences & Neurology |
| WOS类目 | Biochemistry & Molecular Biology ; Neurosciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000235986000021 |
| 出版者 | BLACKWELL PUBLISHING |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379275 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Shen, X |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Grad Sch, Beijing 100101, Peoples R China 2.Shanghai Univ Tradit Chinese Med, Div Nitr Oxide & Inflammatory Med, E Inst Shanghai Univ, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ouyang, MX,Shen, X. Critical role of ask1 in the 6-hydroxydopamine-induced apoptosis in human neuroblastoma sh-sy5y cells[J]. Journal of neurochemistry,2006,97(1):234-244. |
| APA | Ouyang, MX,&Shen, X.(2006).Critical role of ask1 in the 6-hydroxydopamine-induced apoptosis in human neuroblastoma sh-sy5y cells.Journal of neurochemistry,97(1),234-244. |
| MLA | Ouyang, MX,et al."Critical role of ask1 in the 6-hydroxydopamine-induced apoptosis in human neuroblastoma sh-sy5y cells".Journal of neurochemistry 97.1(2006):234-244. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。