Comparative proteomic analysis provides new insights into chilling stress responses in rice
文献类型：期刊论文
| 作者 | Yan, SP; Zhang, QY; Tang, ZC; Su, WA; Sun, WN |
| 刊名 | Molecular & cellular proteomics
 |
| 出版日期 | 2006-03-01 |
| 卷号 | 5期号:3页码:484-496 |
| ISSN号 | 1535-9476 |
| DOI | 10.1074/mcp.m500251-mcp200 |
| 通讯作者 | Sun, wn() |
| 英文摘要 | Low temperature is one of the major abiotic stresses limiting the productivity and the geographical distribution of many important crops. to gain a better understanding of chilling stress responses in rice (oryza sativa l. cv. nipponbare), we carried out a comparative proteomic analysis. three-week-old rice seedlings were treated at 6 degrees c for 6 or 24 h and then recovered for 24 h. chilling treatment resulted in stress phenotypes of rolling leaves, increased relative electrolyte leakage, and decreased net photosynthetic rate. the temporal changes of total proteins in rice leaves were examined using two-dimensional electrophoresis. among similar to 1,000 protein spots reproducibly detected on each gel, 31 protein spots were down-regulated, and 65 were up-regulated at least at one time point. mass spectrometry analysis allowed the identification of 85 differentially expressed proteins, including well known and novel cold-responsive proteins. several proteins showed enhanced degradation during chilling stress, especially the photosynthetic proteins such as rubisco large subunit of which 19 fragments were detected. the identified proteins are involved in several processes, i.e. signal transduction, rna processing, translation, protein processing, redox homeostasis, photosynthesis, photorespiration, and metabolisms of carbon, nitrogen, sulfur, and energy. gene expression analysis of 44 different proteins by quantitative real time pcr showed that the mrna level was not correlated well with the protein level. in conclusion, our study provides new insights into chilling stress responses in rice and demonstrates the advantages of proteomic analysis. |
| WOS关键词 | PLANT COLD-ACCLIMATION ; LENGTH CDNA MICROARRAY ; HIGH-SALINITY STRESSES ; GENE-EXPRESSION ; ABSCISIC-ACID ; RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE ; ARABIDOPSIS GENES ; REDOX HOMEOSTASIS ; OXIDATIVE STRESS ; SALT STRESS |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemical Research Methods |
| 语种 | 英语 |
| WOS记录号 | WOS:000236142800006 |
| 出版者 | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379281 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Sun, WN |
| 作者单位 | 1.Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China 2.Shanghai Med Univ 2, State Key Lab Med Gen, Ruijin Hosp, Shanghai 200025, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan, SP,Zhang, QY,Tang, ZC,et al. Comparative proteomic analysis provides new insights into chilling stress responses in rice[J]. Molecular & cellular proteomics,2006,5(3):484-496. |
| APA | Yan, SP,Zhang, QY,Tang, ZC,Su, WA,&Sun, WN.(2006).Comparative proteomic analysis provides new insights into chilling stress responses in rice.Molecular & cellular proteomics,5(3),484-496. |
| MLA | Yan, SP,et al."Comparative proteomic analysis provides new insights into chilling stress responses in rice".Molecular & cellular proteomics 5.3(2006):484-496. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。