Folding and conformation stability of triose phosphate isomerase
文献类型：期刊论文
| 作者 | Liu, JH; Shi, Y; Zhou, JM |
| 刊名 | Progress in biochemistry and biophysics
 |
| 出版日期 | 2006-05-01 |
| 卷号 | 33期号:5页码:465-472 |
| 关键词 | Triose phosphate isomerase (tim) Denaturation Conformational change Folding intermediate Two-state transition model |
| ISSN号 | 1000-3282 |
| 通讯作者 | Zhou, jm() |
| 英文摘要 | Triose phosphate isomerase (tim) was prepared and purified from chicken breast muscle. the equilibrium unfolding of tim induced by guanidine hydrochloride was investigated by following the changes in intrinsic fluorescence, circular dichroism and second-derivative spectroscopy. results show that the unfolding of tim is highly cooperative and no folding intermediate was detected in the experimental conditions used. the thermodynamic parameters of tim during guanidine denaturation were calculated according to a two-state unimolecular unfolding model. thermal denaturation of tim monitored by cd at 222 nm shows also a single, cooperative transition with an apparent t-m of 64.6 degrees c and the thermal stability of tim was decreased in the presence of low concentrations of guanidine. the possible unfolding pathway of the dimeric tim was discussed. it shows that the secondary and tertiary structural changes of tim occur simultaneously during guanidine denaturation and the unfolding of dimeric tim follows an apparent two-state transition without detectable dissociation model. |
| WOS关键词 | TRIOSEPHOSPHATE ISOMERASE ; CHICKEN-MUSCLE ; EQUILIBRIUM DENATURATION ; RABBIT MUSCLE ; PROTEINS ; HETEROGENEITY ; DEFICIENCY ; SUBUNITS ; EXPOSURE ; KINETICS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000237610400009 |
| 出版者 | SCIENCE CHINA PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379290 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhou, JM |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, JH,Shi, Y,Zhou, JM. Folding and conformation stability of triose phosphate isomerase[J]. Progress in biochemistry and biophysics,2006,33(5):465-472. |
| APA | Liu, JH,Shi, Y,&Zhou, JM.(2006).Folding and conformation stability of triose phosphate isomerase.Progress in biochemistry and biophysics,33(5),465-472. |
| MLA | Liu, JH,et al."Folding and conformation stability of triose phosphate isomerase".Progress in biochemistry and biophysics 33.5(2006):465-472. |
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来源：中国科学院大学
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