Cellular mechanism for spontaneous calcium oscillations in astrocytes
文献类型：期刊论文
| 作者 | Wang, TF; Zhou, C; Tang, AH; Wang, SQ; Chai, Z |
| 刊名 | Acta pharmacologica sinica
 |
| 出版日期 | 2006-07-01 |
| 卷号 | 27期号:7页码:861-868 |
| 关键词 | Hippocampal astrocytes Spontaneous ca2+ oscillations Endoplasmic reticulum ca2+ store Insp(3) receptors Confocal laser scanning microscope |
| ISSN号 | 1671-4083 |
| DOI | 10.1111/j.1745-7254.2006.00397.x |
| 通讯作者 | Chai, z() |
| 英文摘要 | Aim: to determine the ca2+ source and cellular mechanisms of spontaneous ca2+ oscillations in hippocampal astrocytes. methods: the cultured cells were loaded with fluo-4 am, the indicator of intracellular ca2+, and the dynamic ca2+ transients were visualized with confocal laser-scanning microscopy. results: the spontaneous ca2+ oscillations in astrocytes were observed first in co-cultured hippocampal neurons and astrocytes. these oscillations were not affected by tetrodotoxin (ttx) treatment and kept up in purity cultured astrocytes. the spontaneous ca2+ oscillations were not impacted after blocking the voltage-gated ca2+ channels or ethylenediamine tetraacetic acid (edta) bathing, indicating that intracellular ca2+ elevation was not the result of extracellular ca2+ influx. furthermore, the correlation between the spontaneous ca2+ oscillations and the ca2+ store in endoplasmic reticulum (er) were investigated with pharmacological experiments. the oscillations were: 1) enhanced when cells were exposed to both low na+ (70 mmol/l) and high ca2+ (5 mmol/l) solution, and eliminated completely by 2 mu mol/l thapsigargin, a blocker of sarcoplasmic reticulum ca2+-atpase; and 2) still robust after the application with either 50 mu mol/l ryanodine or 400 mu mol/l tetracaine, two specific antagonists of ryanodine receptors, but depressed in a dose-dependent manner by 2-apb, an insp3 receptors (insp(3)r) blocker. conclusion: insp(3)r-induced er ca2+ release is an important cellular mechanism for the initiation of spontaneous ca2+ oscillation in hippocampal astrocytes. |
| WOS关键词 | IN-SITU ; CA2+ OSCILLATIONS ; GLUTAMATE RELEASE ; NEURONAL-ACTIVITY ; GLIAL CALCIUM ; COMMUNICATION ; STORES ; CELLS ; WAVES |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| WOS类目 | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000238485300013 |
| 出版者 | BLACKWELL PUBLISHING |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379343 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chai, Z |
| 作者单位 | Peking Univ, Coll Life Sci, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, TF,Zhou, C,Tang, AH,et al. Cellular mechanism for spontaneous calcium oscillations in astrocytes[J]. Acta pharmacologica sinica,2006,27(7):861-868. |
| APA | Wang, TF,Zhou, C,Tang, AH,Wang, SQ,&Chai, Z.(2006).Cellular mechanism for spontaneous calcium oscillations in astrocytes.Acta pharmacologica sinica,27(7),861-868. |
| MLA | Wang, TF,et al."Cellular mechanism for spontaneous calcium oscillations in astrocytes".Acta pharmacologica sinica 27.7(2006):861-868. |
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来源：中国科学院大学
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