In vitro reassembly of tobacco ribulose-1,5-bisphosphate carboxylase/oxygenase from fully denatured subunits
文献类型：期刊论文
| 作者 | Yong, Zhen-Hua; Chen, Gen-Yun; Shi, Jiao-Nai; Xu, Da-Quan |
| 刊名 | Acta biochimica et biophysica sinica
 |
| 出版日期 | 2006-10-01 |
| 卷号 | 38期号:10页码:737-745 |
| 关键词 | Rubisco Reassembly Gradual dialysis Chaperonin 60 Hsp70 |
| ISSN号 | 1672-9145 |
| DOI | 10.1111/j.1745-7270.2006.00221.x |
| 通讯作者 | Chen, gen-yun(chenggy@sippe.ac.cn) |
| 英文摘要 | It has been generally proved impossible to reassemble ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) from fully denatured subunits in vitro in higher plant, because large subunit of fully denatured rubisco is liable to precipitate when the denaturant is removed by common methods of direct dilution and one-step dialysis. in our experiment, the problem of precipitation was resolved by an improved gradual dialysis method, which gradually decreased the concentration of denaturant. however, fully denatured rubisco subunits still could not be reassembled into holoenzyme using gradual dialysis unless chaperonin 60 was added. the restored activity of reassembled rubisco was approximately 8% of natural enzyme. the quantity of reassembled rubisco increased greatly when heat shock protein 70 was present in the reassembly process. atp and mg2+ were unnecessary for in vitro reassembly of rubisco, and mg2+ inhibited the reassembly process. the reassembly was weakened when atp, mg2+ and k+ existed together in the reassembly process. |
| WOS关键词 | RIBULOSE-BISPHOSPHATE CARBOXYLASE ; SYNTHESIZED LARGE SUBUNITS ; RIBULOSEBISPHOSPHATE CARBOXYLASE ; ESCHERICHIA-COLI ; PROTEIN ; CHAPERONIN ; RECONSTITUTION ; OXYGENASE ; SPINACH ; BINDING |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000241620100010 |
| 出版者 | BLACKWELL PUBLISHING |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379356 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chen, Gen-Yun |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Grad Sch, Shanghai 200032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yong, Zhen-Hua,Chen, Gen-Yun,Shi, Jiao-Nai,et al. In vitro reassembly of tobacco ribulose-1,5-bisphosphate carboxylase/oxygenase from fully denatured subunits[J]. Acta biochimica et biophysica sinica,2006,38(10):737-745. |
| APA | Yong, Zhen-Hua,Chen, Gen-Yun,Shi, Jiao-Nai,&Xu, Da-Quan.(2006).In vitro reassembly of tobacco ribulose-1,5-bisphosphate carboxylase/oxygenase from fully denatured subunits.Acta biochimica et biophysica sinica,38(10),737-745. |
| MLA | Yong, Zhen-Hua,et al."In vitro reassembly of tobacco ribulose-1,5-bisphosphate carboxylase/oxygenase from fully denatured subunits".Acta biochimica et biophysica sinica 38.10(2006):737-745. |
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来源：中国科学院大学
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