Cloning and comparative analysis of the gene encoding diacylglycerol acyltransferase from wild type and cultivated soybean
文献类型：期刊论文
| 作者 | Wang, HW; Zhang, JS; Gai, JY; Chen, SY |
| 刊名 | Theoretical and applied genetics
 |
| 出版日期 | 2006-04-01 |
| 卷号 | 112期号:6页码:1086-1097 |
| 关键词 | Diacylglycerol acyltransferase gene Glycine max Diversity |
| ISSN号 | 0040-5752 |
| DOI | 10.1007/s00122-006-0210-9 |
| 通讯作者 | Zhang, js(sychen@genetics.ac.cn) |
| 英文摘要 | Diacylglycerol acyltransferase (dgat), as an important enzyme in triacylglycerol synthesis, catalyzes the final acylation of the kennedy pathway. in the present study, the gmdgat gene was cloned from glycine max by using atdgat as a query to search against the soybean est database and the rapid amplification of cdna ends (race) method. allelic genes were also isolated from 13 soybean accessions and the divergence of the deduced amino acid sequences were compared. the comparison reveals that although gmdgat is a highly conserved protein, several differences of insertion/deletion were identified in the n-terminal region of the gmdgats from various soybean accessions. in the c-terminal regions, a single amino acid mutation specific to both g. max and g. soja was also found. the gmdgat genomic sequences were further cloned and the number and size of exons in the dgat genomic sequence were very similar among different plant species, whereas the introns were more diverged. these results may have significance in elucidating the genetic diversity of the gmdgat among the soybean subgenus. |
| WOS关键词 | ARABIDOPSIS-THALIANA ; ENDOPLASMIC-RETICULUM ; DEVELOPING SEEDS ; BRASSICA-NAPUS ; EXPRESSION ; GLYCINE ; BIOSYNTHESIS ; PROTEIN ; ENZYME ; MUTANT |
| WOS研究方向 | Agriculture ; Plant Sciences ; Genetics & Heredity |
| WOS类目 | Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture |
| 语种 | 英语 |
| WOS记录号 | WOS:000236486600009 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379362 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhang, JS |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Nanjing Univ, Natl Soybean Inst Improvement, Nanjing 210095, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, HW,Zhang, JS,Gai, JY,et al. Cloning and comparative analysis of the gene encoding diacylglycerol acyltransferase from wild type and cultivated soybean[J]. Theoretical and applied genetics,2006,112(6):1086-1097. |
| APA | Wang, HW,Zhang, JS,Gai, JY,&Chen, SY.(2006).Cloning and comparative analysis of the gene encoding diacylglycerol acyltransferase from wild type and cultivated soybean.Theoretical and applied genetics,112(6),1086-1097. |
| MLA | Wang, HW,et al."Cloning and comparative analysis of the gene encoding diacylglycerol acyltransferase from wild type and cultivated soybean".Theoretical and applied genetics 112.6(2006):1086-1097. |
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来源：中国科学院大学
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