Inducible and reversible suppression of npm1 gene expression using stably integrated small interfering rna vector in mouse embryonic stem cells
文献类型：期刊论文
| 作者 | Wang, Bei Bei; Lu, Rui; Wang, Wei Cheng; Jin, Ying |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2006-09-08 |
| 卷号 | 347期号:4页码:1129-1137 |
| 关键词 | Rna interference Inducible Embryonic stem cells Nucleophosmin Proliferation |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2006.07.020 |
| 通讯作者 | Jin, ying(yjin@sibs.ac.cn) |
| 英文摘要 | The tetracycline (tc)-inducible small interference rna (sirna) is a powerful toot for studying gene function in mammalian cells. however, the system is infrequently utilized in embryonic stem (es) cells. here, we present the first application of the tc-inducible, stably integrated plasmid-based sirna system in mouse es cells to down-regulate expression of npm1, an essential gene for embryonic development. the physiological role of npm1 in es cells has not been defined. our data show that the knock-down of npm1 expression by this sirna system was not only highly efficient, but also tc-dose- and induction time-dependent. particularly, the down-regulation of npm1 expression was reversible. importantly, suppression of npm1 expression in es cells resulted in reduced cell proliferation. taken together, this. system allows for studying gene function in a highly controlled manner, otherwise difficult to achieve in es cells. moreover, our results demonstrate that npm1 is essential for es cell proliferation. (c) 2006 elsevier inc. all rights reserved. |
| WOS关键词 | MAMMALIAN-CELLS ; ES CELLS ; NUCLEOPHOSMIN/B23 ; DIFFERENTIATION ; KNOCKDOWN ; CLONING ; GROWTH |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000239681100040 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379373 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Jin, Ying |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Hlth Sci, Key Lab Stem Cell Biol, Shanghai 200025, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Med, Lab Mol Dev Biol, Shanghai 200025, Peoples R China 3.Shanghai Jiao Tong Univ, Sch Med, Chinese Minist Educ, Key Lab Cell Differentiat & Apoptosis, Shanghai 200025, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Bei Bei,Lu, Rui,Wang, Wei Cheng,et al. Inducible and reversible suppression of npm1 gene expression using stably integrated small interfering rna vector in mouse embryonic stem cells[J]. Biochemical and biophysical research communications,2006,347(4):1129-1137. |
| APA | Wang, Bei Bei,Lu, Rui,Wang, Wei Cheng,&Jin, Ying.(2006).Inducible and reversible suppression of npm1 gene expression using stably integrated small interfering rna vector in mouse embryonic stem cells.Biochemical and biophysical research communications,347(4),1129-1137. |
| MLA | Wang, Bei Bei,et al."Inducible and reversible suppression of npm1 gene expression using stably integrated small interfering rna vector in mouse embryonic stem cells".Biochemical and biophysical research communications 347.4(2006):1129-1137. |
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来源：中国科学院大学
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