The effect of collagen-targeting platelet-derived growth factor on cellularization and vascularization of collagen scaffolds
文献类型：期刊论文
| 作者 | Lin, Hang; Chen, Bing; Sun, Wenjie; Zhao, Wenxue; Zhao, Yannan; Dai, Jianwu |
| 刊名 | Biomaterials
 |
| 出版日期 | 2006-11-01 |
| 卷号 | 27期号:33页码:5708-5714 |
| 关键词 | Platelet-derived growth factor bb (pdgf-bb) Collagen Collagen-targeting system Collagen-binding domain (cbd) |
| ISSN号 | 0142-9612 |
| DOI | 10.1016/j.biomaterials.2006.07.023 |
| 通讯作者 | Dai, jianwu(jwdai@genetics.ac.cn) |
| 英文摘要 | Platelet-derived growth factor bb (pdgf-bb) was an important inductive factor during wound healing, but the lack of efficient delivery system limited its clinical application. here, a peptide of seven amino acids was firstly utilized to engineer pdgf-bb to construct a collagen-targeting system. this peptide functioned as collagen-binding domain (cbd) to specially target the pdgf-bb to the collagen and restrict its diffusion. in our study, in vitro activity assay showed that the cbd-fused pdgf (cbd-pdgf) and native pdgf (nat-pdgf) possessed similar activity to stimulate the human fibroblast proliferation. as expected, this peptide promoted the binding of pdgf to collagen scaffolds, and cbd-pdgf caused more cells to proliferate on the collagen gel than nat-pdgf when the same amounts of pdgf were added. in the in vivo experiment, cbd-pdgf-loaded collagen scaffolds were uniformly cellularized and vascularized compared to that of nat-pdgf-loaded scaffolds. our study demonstrated that the cbd targeted pdgf to the collagen scaffold to exert its functions, and it suggested this could be an effective system for targeting tissue regeneration and wound repair. (c) 2006 elsevier ltd. all rights reserved. |
| WOS关键词 | FACTOR FUSION PROTEIN ; FACTOR DELIVERY ; BINDING DOMAIN ; RENATURATION ; OPPORTUNITIES ; EXPRESSION ; CHALLENGES ; TISSUES ; MATRIX ; MODEL |
| WOS研究方向 | Engineering ; Materials Science |
| WOS类目 | Engineering, Biomedical ; Materials Science, Biomaterials |
| 语种 | 英语 |
| WOS记录号 | WOS:000241131500010 |
| 出版者 | ELSEVIER SCI LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379389 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Dai, Jianwu |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Mol Dev Biol, Inst Genet & Dev Biol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, Hang,Chen, Bing,Sun, Wenjie,et al. The effect of collagen-targeting platelet-derived growth factor on cellularization and vascularization of collagen scaffolds[J]. Biomaterials,2006,27(33):5708-5714. |
| APA | Lin, Hang,Chen, Bing,Sun, Wenjie,Zhao, Wenxue,Zhao, Yannan,&Dai, Jianwu.(2006).The effect of collagen-targeting platelet-derived growth factor on cellularization and vascularization of collagen scaffolds.Biomaterials,27(33),5708-5714. |
| MLA | Lin, Hang,et al."The effect of collagen-targeting platelet-derived growth factor on cellularization and vascularization of collagen scaffolds".Biomaterials 27.33(2006):5708-5714. |
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来源：中国科学院大学
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