Isolation and characterization of a sodium-dependent phosphate transporter gene in dunaliella viridis
文献类型：期刊论文
| 作者 | Li, QY; Gao, XS; Sun, Y; Zhang, QQ; Song, RT; Xu, ZK |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2006-02-03 |
| 卷号 | 340期号:1页码:95-104 |
| 关键词 | Dunaliella viridis Sodium-dependent phosphate transporter Induced expression Yeast functional characterization |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2005.11.144 |
| 通讯作者 | Xu, zk() |
| 英文摘要 | A sodium-dependent phosphate transporter gene, dvspt1, was isolated from a cdna library using a probe derived from a subtracted cdna library of dunaliella viridis. sequencing analyses revealed a cdna-sequence of 2649 bp long and encoded an open-reading frame consisting of 672 amino acids. the deduced amino acid sequence of dvspt1 exhibited 31.2% identity to that of tcpho from tetraselmis chui. hydrophobicity and secondary structure prediction revealed 11 conserved transmembrane domains similar to those found in pho89 from saccharomyces cerevisiae and pho4 from neurospora crassa. northern blot analysis indicated that the dvspti expression was induced upon nacl hyperosmotic stress or phosphate depletion. functional characterization in yeast na+ export pump mutant g19 suggested that dvspt1 encoded a na+ transporter protein. the gene sequence of gdvspt1 (7922 bp) was isolated from a genomic library of d. viridis. southern blot analysis indicated that there exist at least two homologous genes in d. viridis. (c) 2005 elsevier inc. all rights reserved. |
| WOS关键词 | HALOTOLERANT ALGA DUNALIELLA ; SACCHAROMYCES-CEREVISIAE ; POLYPHOSPHATE-HYDROLYSIS ; ALKALINE STRESS ; PROTEIN ; CHLAMYDOMONAS ; EXPRESSION ; SEQUENCE ; SALINA ; TRANSCRIPTION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000234640600015 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379396 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Xu, ZK |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 10039, Peoples R China 3.Shanghai Univ, Sch Life Sci, Shanghai 200444, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, QY,Gao, XS,Sun, Y,et al. Isolation and characterization of a sodium-dependent phosphate transporter gene in dunaliella viridis[J]. Biochemical and biophysical research communications,2006,340(1):95-104. |
| APA | Li, QY,Gao, XS,Sun, Y,Zhang, QQ,Song, RT,&Xu, ZK.(2006).Isolation and characterization of a sodium-dependent phosphate transporter gene in dunaliella viridis.Biochemical and biophysical research communications,340(1),95-104. |
| MLA | Li, QY,et al."Isolation and characterization of a sodium-dependent phosphate transporter gene in dunaliella viridis".Biochemical and biophysical research communications 340.1(2006):95-104. |
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来源：中国科学院大学
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