Development of enzyme biosensor for amperometric measurement of aspartate aminotransferase
文献类型：期刊论文
| 作者 | Guo, Zonghui; Liu, Chunxiu; Li, Huaqing; Xu, Jianfang; Feng, Lianshi; Cai, Xinxia |
| 刊名 | Rare metal materials and engineering
 |
| 出版日期 | 2006-12-01 |
| 卷号 | 35页码:330-332 |
| 关键词 | Aspartate aminotransferase Redox polymer Amperometric Biosensor |
| ISSN号 | 1002-185X |
| 通讯作者 | Cai, xinxia(xxcai@mail.ie.ac.cn) |
| 英文摘要 | An enzyme biosensor for amperometric measurement of aspartate aminotransferase has been developed. the working electrode was modified with a thin-film of redox polymer, then glutamate oxidase, with the immobilized reagent cast and dried on the electrode. the biosensor responses to ast by detecting. hydrogen peroxide were produced by enzymical reaction at -0.1 v with a response time of 120 seconds. the electrode gave a detection limit of 32.5 u/l with a linear concentration range of 32.5 u/l similar to 2000 u/l in serum. due to more sensitive and lower detection limit, the biosensor is expected mainly to be used for physiological identification and physical performance of athletes in the future. extended application will also affect the practice of clinical medicine for the diagnosis of heart and liver disease. |
| WOS研究方向 | Materials Science ; Metallurgy & Metallurgical Engineering |
| WOS类目 | Materials Science, Multidisciplinary ; Metallurgy & Metallurgical Engineering |
| 语种 | 英语 |
| WOS记录号 | WOS:000244457000105 |
| 出版者 | NORTHWEST INST NONFERROUS METAL RESEARCH |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379399 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Cai, Xinxia |
| 作者单位 | 1.Chinese Acad Sci, Inst Elect, State Key Lab Transducer Technol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China 3.China Inst Sport Sci, Ctr Biol, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Zonghui,Liu, Chunxiu,Li, Huaqing,et al. Development of enzyme biosensor for amperometric measurement of aspartate aminotransferase[J]. Rare metal materials and engineering,2006,35:330-332. |
| APA | Guo, Zonghui,Liu, Chunxiu,Li, Huaqing,Xu, Jianfang,Feng, Lianshi,&Cai, Xinxia.(2006).Development of enzyme biosensor for amperometric measurement of aspartate aminotransferase.Rare metal materials and engineering,35,330-332. |
| MLA | Guo, Zonghui,et al."Development of enzyme biosensor for amperometric measurement of aspartate aminotransferase".Rare metal materials and engineering 35(2006):330-332. |
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来源：中国科学院大学
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