Direct organogenesis and plantlet regeneration from mature zygotic embryos of masson pine (pinus massoniana l.)
文献类型：期刊论文
| 作者 | Zhang, Y; Wei, ZM; Xi, ML; Shi, JS |
| 刊名 | Plant cell tissue and organ culture
 |
| 出版日期 | 2006 |
| 卷号 | 84期号:1页码:119-123 |
| 关键词 | Adventious bud induction Histology Micropropagation Rooting Shoot regeneration |
| ISSN号 | 0167-6857 |
| DOI | 10.1007/s11240-005-9004-z |
| 通讯作者 | Wei, zm() |
| 英文摘要 | Mature zygotic embryos of masson pine were cultured as initial explants to investigate the process of direct organogenesis. adventitious buds were initiated on dcr medium (douglas-fir cotyledon revised medium) supplemented with 0.5 mg l(-1) n-6-benzyladenine (ba) and 0.05 mg l(-1) indolebutyric acid (iba) or alpha-naphthaleneacetic acid (naa). the highest induction frequency of adventitious buds was 99.3%. subsequent transfer of buds to medium with lower concentrations of plant growth regulators in time was necessary for differentation of high quality adventitious buds. after culturing on elongating medium, in which the proportion of cytokinins to auxins was reduced, shoots higher than 2 cm were transferred for root induction to gd medium with half of the concentration of macro-salts (1/2 gd) and with 2 mg l(-1) iba and 0.05 mg l(-1) ba. the average root frequency was over 70%. after adventitious roots had appeared, the shoots were transferred to 1/2 gd medium with a lower concentration of iba (0.2 mg l(-1)) for further root development. |
| WOS关键词 | DIFFERENTIATION ; RADIATA ; MILL |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Plant Sciences |
| WOS类目 | Biotechnology & Applied Microbiology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000236371200012 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379452 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wei, ZM |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Grad Sch, Shanghai 200032, Peoples R China 3.Nanjing Forestry Univ, Key Lab Forestry Genet & Gene Engn, Nanjing 210037, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Y,Wei, ZM,Xi, ML,et al. Direct organogenesis and plantlet regeneration from mature zygotic embryos of masson pine (pinus massoniana l.)[J]. Plant cell tissue and organ culture,2006,84(1):119-123. |
| APA | Zhang, Y,Wei, ZM,Xi, ML,&Shi, JS.(2006).Direct organogenesis and plantlet regeneration from mature zygotic embryos of masson pine (pinus massoniana l.).Plant cell tissue and organ culture,84(1),119-123. |
| MLA | Zhang, Y,et al."Direct organogenesis and plantlet regeneration from mature zygotic embryos of masson pine (pinus massoniana l.)".Plant cell tissue and organ culture 84.1(2006):119-123. |
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来源：中国科学院大学
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