Characterization of drug metabolites and cytotoxicity assay simultaneously using an integrated microfluidic device
文献类型：期刊论文
| 作者 | Ma, Bo1,2; Zhang, Guohao1,2; Qin, Jianhua1; Lin, Bingcheng1 |
| 刊名 | lab on a chip
 |
| 出版日期 | 2009 |
| 卷号 | 9期号:2页码:232-238 |
| 产权排序 | 1;1 |
| 通讯作者 | 秦建华 ; 林炳承 |
| 英文摘要 | an integrated microfluidic device was developed for the characterization of drug metabolites and a cytotoxicity assay simultaneously. the multi-layer device was composed of a quartz substrate with embedded separation microchannels and a perforated three-microwell array containing sol-gel bioreactors of human liver microsome (hlm), and two pdms layers. by aligning the microwell array on the quartz substrate with cell culture chambers on the bottom pdms layer, drug metabolism studies related to functional units, including metabolite generation, detection and incubation with cultured cells to assess metabolism induced cytotoxicity, were all integrated into the microfluidic device. to validate the feasibility of drug metabolism study on the microfluidic chip, udp-glucuronosyltransferase (ugt) metabolism of acetaminophen (ap) and its effect on hepg2 cytotoxicity were studied first. then metabolism based drug-drug interaction between ap and phenytoin (ph), which resulted in increased hepg2 cytotoxicity, was proved on this device. all this demonstrated that the developed microfluidic device could be a potential useful tool for drug metabolism and metabolism based drug-drug interaction research. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, multidisciplinary ; nanoscience & nanotechnology |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry ; science & technology - other topics |
| 关键词[WOS] | uv absorbency detection ; udp-glucuronosyltransferases ; induced hepatotoxicity ; toxicity ; chip ; microreactor ; hepatocytes ; kinetics ; arrays ; phase |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000262649500007 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/101393]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Bo,Zhang, Guohao,Qin, Jianhua,et al. Characterization of drug metabolites and cytotoxicity assay simultaneously using an integrated microfluidic device[J]. lab on a chip,2009,9(2):232-238. |
| APA | Ma, Bo,Zhang, Guohao,Qin, Jianhua,&Lin, Bingcheng.(2009).Characterization of drug metabolites and cytotoxicity assay simultaneously using an integrated microfluidic device.lab on a chip,9(2),232-238. |
| MLA | Ma, Bo,et al."Characterization of drug metabolites and cytotoxicity assay simultaneously using an integrated microfluidic device".lab on a chip 9.2(2009):232-238. |
入库方式： OAI收割
来源：大连化学物理研究所
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