Anti-cd146 monoclonal antibody aa98 inhibits angiogenesis via suppression of nuclear factor-kappa b activation
文献类型：期刊论文
| 作者 | Bu, Pengcheng; Gao, Lizeng; Zhuang, Jie; Feng, Jing; Yang, Dongling; Yan, Xiyun |
| 刊名 | Molecular cancer therapeutics
 |
| 出版日期 | 2006-11-01 |
| 卷号 | 5期号:11页码:2872-2878 |
| ISSN号 | 1535-7163 |
| DOI | 10.1158/1535-7163.mct-06-0260 |
| 通讯作者 | Yan, xiyun(yanxy@sun5.ibp.ac.cn) |
| 英文摘要 | Our previous study showed that an anti-cd146 monoclonal antibody (mab), aa98, which was raised against the vascular endothelial cells stimulated by a conditioned medium from hepatocarcinoma smmc 7721 cells (smmc 7721-cm), inhibited cell migration, angiogenesis, and tumor growth. however, the underlying mechanism was not elucidated. the objective of this study was to understand the mechanism by which mab aa98 inhibits the endothelial cell migration and angiogenesis that is induced by smmc 7721-cm. using confocal imaging and biochemical studies, we found that smmc 7721-cm induced nuclear factor kappa b (nf-kappa b) activation through the upstream p38 mitogen-activated protein kinase pathway, leading to the up-regulation of matrix metalloproteinase 9 and intercellular adhesion molecule 1 expression. interestingly, all these activities stimulated by smmc 7721-cm could be effectively inhibited by mab aa98 in a dose- and time-dependent manner. our data showed that the engagement of mab aa98 with membrane protein cd146 inhibited p38 mitogen-activated protein kinase phosphorylation, suppressed nf-kappa b activation, and down-regulated matrix metalloproteinase 9 and intercellular adhesion molecule 1 expression, suggesting that the suppression of nf-kb is a critical point for the inhibitory function of mab aa98 on endothelial cell migration, angiogenesis, and tumor metastasis. these results will provide clues for a better understanding of the mechanisms underlying tumor angiogenesis as well as antiangiogenesis therapy. |
| WOS关键词 | HUMAN ENDOTHELIAL-CELLS ; HUMAN-MELANOMA ; TUMOR-GROWTH ; P38 MAPK ; EXPRESSION ; METASTASIS ; CARCINOMA ; PROTEIN ; MATRIX-METALLOPROTEINASE-9 ; IDENTIFICATION |
| WOS研究方向 | Oncology |
| WOS类目 | Oncology |
| 语种 | 英语 |
| WOS记录号 | WOS:000242138000029 |
| 出版者 | AMER ASSOC CANCER RESEARCH |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379472 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yan, Xiyun |
| 作者单位 | 1.Chinese Acad Sci, Natl Lab Biomicromol, Inst Biophys, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bu, Pengcheng,Gao, Lizeng,Zhuang, Jie,et al. Anti-cd146 monoclonal antibody aa98 inhibits angiogenesis via suppression of nuclear factor-kappa b activation[J]. Molecular cancer therapeutics,2006,5(11):2872-2878. |
| APA | Bu, Pengcheng,Gao, Lizeng,Zhuang, Jie,Feng, Jing,Yang, Dongling,&Yan, Xiyun.(2006).Anti-cd146 monoclonal antibody aa98 inhibits angiogenesis via suppression of nuclear factor-kappa b activation.Molecular cancer therapeutics,5(11),2872-2878. |
| MLA | Bu, Pengcheng,et al."Anti-cd146 monoclonal antibody aa98 inhibits angiogenesis via suppression of nuclear factor-kappa b activation".Molecular cancer therapeutics 5.11(2006):2872-2878. |
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来源：中国科学院大学
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