Cze determination of flavonoids in hatenia elliptica
文献类型：期刊论文
| 作者 | Yang, HP; Yue, ME; Shi, YP |
| 刊名 | Chromatographia
 |
| 出版日期 | 2006-05-01 |
| 卷号 | 63期号:9-10页码:449-452 |
| 关键词 | Capillary electropkoresis Pharmaceutical analysis Flavonoid Halenio elliptica Gentianaceae |
| ISSN号 | 0009-5893 |
| DOI | 10.1365/s10337-006-0791-0 |
| 通讯作者 | Shi, yp() |
| 英文摘要 | A capillary zone electrophoretic method with uv detection at 254 nm has been developed and validated for quantitative analysis of three flavonoids, luteolin-7-o-beta-d-glycoside, genkwanin, and luteolin, in halenio elliptica. the applied voltage was 15 kv and the capillary temperature was kept constant at 25 degrees c. the effects on migration of buffer ph, electrolyte concentration, and organic modifier were studied systematically. optimum separation was achieved with 20 mm borate buffer at ph 9.00 containing 10% (v/v) acetonitrile. regression equations revealed a good linear relationship between the peak area of each compound and its concentration. all correlation coefficients were 0.9999. the relative standard deviations of migration time and peak areas were, respectively, < 1.58 and 5.02% (intra-day) and < 1.69% and 5.36% (inter-day). the amounts of the three compounds in the different parts of h. elliptica (stems, leaves, and flowers) and in the extracts of the flowers obtained with different solvents (100% methanol and 50% and 75% methanol in water) were successfully determined with satisfactory repeatability and recovery. |
| WOS关键词 | CAPILLARY-ZONE-ELECTROPHORESIS ; SEPARATION ; GLYCOSIDE ; ACIDS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000237841500008 |
| 出版者 | VIEWEG |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379473 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Shi, YP |
| 作者单位 | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, HP,Yue, ME,Shi, YP. Cze determination of flavonoids in hatenia elliptica[J]. Chromatographia,2006,63(9-10):449-452. |
| APA | Yang, HP,Yue, ME,&Shi, YP.(2006).Cze determination of flavonoids in hatenia elliptica.Chromatographia,63(9-10),449-452. |
| MLA | Yang, HP,et al."Cze determination of flavonoids in hatenia elliptica".Chromatographia 63.9-10(2006):449-452. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。