Expression profiling of the 14-3-3 gene family in response to salt stress and potassium and iron deficiencies in young tomato (solanum lycopersicum) roots: analysis by real-time rt-pcr
文献类型：期刊论文
| 作者 | Xu, Wei Feng; Shi, Wei Ming |
| 刊名 | Annals of botany
 |
| 出版日期 | 2006-11-01 |
| 卷号 | 98期号:5页码:965-974 |
| 关键词 | Solanum lycopersicum Real-time rt-pcr Expression Gene family 14-3-3 Salt stress and potassium deficiency Iron deficiency |
| ISSN号 | 0305-7364 |
| DOI | 10.1093/aob/mcl189 |
| 通讯作者 | Shi, wei ming(wmshi@issas.ac.cn) |
| 英文摘要 | Background and aims mineral nutrient deficiencies and salinity constitute major limitations for crop plant growth on agricultural soils. 14-3-3 proteins are phosphoserine-binding proteins that regulate the activities of a wide array of targets via direct protein-protein interactions and may play an important role in responses to mineral nutrients deficiencies and salt stress. in the present study, the expression profiling of the 14-3-3 gene family in response to salt stress and potassium and iron deficiencies in young tomato (solanum lycopersicum) roots was investigated in order to analyse the 14-3-3 roles of the proteins in these abiotic stresses. methods sequence identities and phylogenetic tree creation were performed using dnaman version 4.0 (lynnon biosoft company). real-time rt-pcr was used to examine the expression of each 14-3-3 gene in response to salt stress and potassium and iron deficiencies in young tomato roots. key results the phylogenetic tree shows that the 14-3-3 gene family falls into two major groups in tomato plants. by using real-time rt-pcr, it was found that (a) under normal growth conditions, there were significant differences in the mrna levels of 14-3-3 gene family members in young tomato roots and (b) 14-3-3 proteins exhibited diverse patterns of gene expression in response to salt stress and potassium and iron deficiencies in tomato roots. conclusions the results suggest that (a) 14-3-3 proteins may be involved in the salt stress and potassium and iron deficiency signalling pathways in young tomato roots, (b) the expression pattern of 14-3-3 gene family members in tomato roots is not strictly related to the position of the corresponding proteins within a phylogenetic tree, (c) gene-specific expression patterns indicate that isoform-specificity may exist in the 14-3-3 gene family of tomato roots, and (d) 14-3-3 proteins (tft7) might mediate cross-talk between the salt stress and potassium and iron-deficiency signalling pathways in tomato roots. |
| WOS关键词 | MEMBRANE H+-ATPASE ; PLASMA-MEMBRANE ; GENE-EXPRESSION ; ISOFORM SPECIFICITY ; ASCORBATE PEROXIDASE ; PROTEIN-REGULATION ; HIGHER-PLANTS ; C-TERMINUS ; CROSS-TALK ; ARABIDOPSIS |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000241629500006 |
| 出版者 | OXFORD UNIV PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379475 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Shi, Wei Ming |
| 作者单位 | 1.Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100081, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Wei Feng,Shi, Wei Ming. Expression profiling of the 14-3-3 gene family in response to salt stress and potassium and iron deficiencies in young tomato (solanum lycopersicum) roots: analysis by real-time rt-pcr[J]. Annals of botany,2006,98(5):965-974. |
| APA | Xu, Wei Feng,&Shi, Wei Ming.(2006).Expression profiling of the 14-3-3 gene family in response to salt stress and potassium and iron deficiencies in young tomato (solanum lycopersicum) roots: analysis by real-time rt-pcr.Annals of botany,98(5),965-974. |
| MLA | Xu, Wei Feng,et al."Expression profiling of the 14-3-3 gene family in response to salt stress and potassium and iron deficiencies in young tomato (solanum lycopersicum) roots: analysis by real-time rt-pcr".Annals of botany 98.5(2006):965-974. |
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来源：中国科学院大学
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