The expression, purification and crystallization of monomeric soluble human urokinase receptor
文献类型：期刊论文
| 作者 | Yuan, C; Huai, Q; Bian, CB; Huang, MD |
| 刊名 | Progress in biochemistry and biophysics
 |
| 出版日期 | 2006-03-01 |
| 卷号 | 33期号:3页码:277-281 |
| 关键词 | Soluble human urokinase receptor S2 cells Purification Crystallization |
| ISSN号 | 1000-3282 |
| 通讯作者 | Huang, md() |
| 英文摘要 | Soluble form of human urokinase receptor was cloned into drosophila schneider 2 secretion expression vector pmt/bip/v5-his, and co-transfected with pcohygro into s2 cells, to establish stably s2 cells line. the expressed supar from such procedure tends to form oligomer and is unstable, presenting difficulties for its structural studies. here a purification procedure that yields monomeric supar was reported. supar obtained through such procedure is monomeric and quite stable. supar complex with amino terminal fragment (atf) of upa and an anti-supar antibody (atn615) was crystallized by dialysis method into diffracting crystals (1.9 angstrom). |
| WOS关键词 | PLASMINOGEN-ACTIVATOR ; LOCALIZATION ; COMPLEX ; DOMAIN |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000236134000011 |
| 出版者 | SCIENCE PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379528 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Huang, MD |
| 作者单位 | 1.Chinese Acad Sci, Fujian Inst Res Struct Matter, State Key Lab Struct Chem, Fujian 350002, Peoples R China 2.Harvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Ctr Haemostasis & Thrombosis Res, Boston, MA 02215 USA 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yuan, C,Huai, Q,Bian, CB,et al. The expression, purification and crystallization of monomeric soluble human urokinase receptor[J]. Progress in biochemistry and biophysics,2006,33(3):277-281. |
| APA | Yuan, C,Huai, Q,Bian, CB,&Huang, MD.(2006).The expression, purification and crystallization of monomeric soluble human urokinase receptor.Progress in biochemistry and biophysics,33(3),277-281. |
| MLA | Yuan, C,et al."The expression, purification and crystallization of monomeric soluble human urokinase receptor".Progress in biochemistry and biophysics 33.3(2006):277-281. |
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来源：中国科学院大学
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