中国科学院机构知识库网格
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m-Aminophenylboronic acid as a functional monomer for fabricating molecularly imprinted polymer for the recognition of bovine serum albumin

文献类型:期刊论文

作者Wang, Huafang1; Li, Wenyou1; He, Xiwen1; Chen, Langxing1; Zhang, Yukui1,2
刊名reactive & functional polymers
出版日期2008-09-01
卷号68期号:9页码:1291-1296
关键词molecularly imprinted polymer m-Aminophenylboronic acid protein adsorption capacity bovine serum albumin (BSA)
产权排序1;1
通讯作者he ; xiwen
英文摘要using m-aminophenyl boronic acid (apba) as a functional monomer, molecularly imprinted polymer (mip) imprinted with bovine serum albumin (bsa) was fabricated on activated glass spheres under optimized conditions. key factors in the prepolymerization reaction (between apba and bsa), such as buffer ph, ionic strength and reaction time, were carefully optimized as previously reported [h.f. wang, w.y. li, x. w. he, et al. acta chim. sinica 65 (2007) 43-48]. the interaction between apba and bsa during the prepolymerization stage was investigated and optimized, and ideal conditions for protein rebinding experiments were determined as well. protein rebinding and enriching properties of polymers were studied in single and competitive binding protocols, respectively. the key point of the present paper is that the binding selectivity of polymers may be estimated by the amount of bound-protein recovered from a protein-saturated polymer. results demonstrated that the selectivity of mip towards its template protein is superior to that of non-imprinted polymer (nip). (c) 2008 elsevier ltd. all rights reserved.
WOS标题词science & technology ; physical sciences ; technology
类目[WOS]chemistry, applied ; engineering, chemical ; polymer science
研究领域[WOS]chemistry ; engineering ; polymer science
关键词[WOS]chitosan beads ; adsorptive separation ; selective separation ; protein recognition ; ionic-strength ; hemoglobin ; antibodies ; particles ; binding ; sites
收录类别SCI
原文出处true
语种英语
WOS记录号WOS:000259655400001
公开日期2010-11-30
源URL[http://159.226.238.44/handle/321008/101415]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
作者单位1.Nankai Univ, Dept Chem, Minist Educ China, Key Lab Funct Polymer Mat, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
推荐引用方式
GB/T 7714
Wang, Huafang,Li, Wenyou,He, Xiwen,et al. m-Aminophenylboronic acid as a functional monomer for fabricating molecularly imprinted polymer for the recognition of bovine serum albumin[J]. reactive & functional polymers,2008,68(9):1291-1296.
APA Wang, Huafang,Li, Wenyou,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2008).m-Aminophenylboronic acid as a functional monomer for fabricating molecularly imprinted polymer for the recognition of bovine serum albumin.reactive & functional polymers,68(9),1291-1296.
MLA Wang, Huafang,et al."m-Aminophenylboronic acid as a functional monomer for fabricating molecularly imprinted polymer for the recognition of bovine serum albumin".reactive & functional polymers 68.9(2008):1291-1296.

入库方式: OAI收割

来源:大连化学物理研究所

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