m-Aminophenylboronic acid as a functional monomer for fabricating molecularly imprinted polymer for the recognition of bovine serum albumin
文献类型:期刊论文
作者 | Wang, Huafang1; Li, Wenyou1; He, Xiwen1; Chen, Langxing1; Zhang, Yukui1,2 |
刊名 | reactive & functional polymers
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出版日期 | 2008-09-01 |
卷号 | 68期号:9页码:1291-1296 |
关键词 | molecularly imprinted polymer m-Aminophenylboronic acid protein adsorption capacity bovine serum albumin (BSA) |
产权排序 | 1;1 |
通讯作者 | he ; xiwen |
英文摘要 | using m-aminophenyl boronic acid (apba) as a functional monomer, molecularly imprinted polymer (mip) imprinted with bovine serum albumin (bsa) was fabricated on activated glass spheres under optimized conditions. key factors in the prepolymerization reaction (between apba and bsa), such as buffer ph, ionic strength and reaction time, were carefully optimized as previously reported [h.f. wang, w.y. li, x. w. he, et al. acta chim. sinica 65 (2007) 43-48]. the interaction between apba and bsa during the prepolymerization stage was investigated and optimized, and ideal conditions for protein rebinding experiments were determined as well. protein rebinding and enriching properties of polymers were studied in single and competitive binding protocols, respectively. the key point of the present paper is that the binding selectivity of polymers may be estimated by the amount of bound-protein recovered from a protein-saturated polymer. results demonstrated that the selectivity of mip towards its template protein is superior to that of non-imprinted polymer (nip). (c) 2008 elsevier ltd. all rights reserved. |
WOS标题词 | science & technology ; physical sciences ; technology |
类目[WOS] | chemistry, applied ; engineering, chemical ; polymer science |
研究领域[WOS] | chemistry ; engineering ; polymer science |
关键词[WOS] | chitosan beads ; adsorptive separation ; selective separation ; protein recognition ; ionic-strength ; hemoglobin ; antibodies ; particles ; binding ; sites |
收录类别 | SCI |
原文出处 | true |
语种 | 英语 |
WOS记录号 | WOS:000259655400001 |
公开日期 | 2010-11-30 |
源URL | [http://159.226.238.44/handle/321008/101415] ![]() |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
作者单位 | 1.Nankai Univ, Dept Chem, Minist Educ China, Key Lab Funct Polymer Mat, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Huafang,Li, Wenyou,He, Xiwen,et al. m-Aminophenylboronic acid as a functional monomer for fabricating molecularly imprinted polymer for the recognition of bovine serum albumin[J]. reactive & functional polymers,2008,68(9):1291-1296. |
APA | Wang, Huafang,Li, Wenyou,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2008).m-Aminophenylboronic acid as a functional monomer for fabricating molecularly imprinted polymer for the recognition of bovine serum albumin.reactive & functional polymers,68(9),1291-1296. |
MLA | Wang, Huafang,et al."m-Aminophenylboronic acid as a functional monomer for fabricating molecularly imprinted polymer for the recognition of bovine serum albumin".reactive & functional polymers 68.9(2008):1291-1296. |
入库方式: OAI收割
来源:大连化学物理研究所
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