A new quasi-interpenetrating network formed by poly(n-acryloyl-tris(hydroxymethyl)aminomethane and polyvinylpyrrolidone: separation matrix for double-stranded dna and single-stranded dna fragments by capillary electrophoresis with uv detection
文献类型：期刊论文
| 作者 | Wang, Q; Xu, X; Dai, LX |
| 刊名 | Electrophoresis
 |
| 出版日期 | 2006-05-01 |
| 卷号 | 27期号:9页码:1749-1757 |
| 关键词 | Capillary electrophoresis Non-gel sieving Poly(n-acryloyl-tris(hydroxymethyl)aminomethane Polyvinylpyrroliclone Quasi-interpenetrating network |
| ISSN号 | 0173-0835 |
| DOI | 10.1002/elps.200500597 |
| 通讯作者 | Xu, x() |
| 英文摘要 | The preparation of a new separation matrix, quasi-interpenetrating networks (quasi-ipns) formed by poly(n-acryloyl-tris) (poly(tris-a)) and pvp, and its application for dsdna and ssdna fragments separation by ce with uv detection, are presented. this new quasi-ipn exhibited high sieving performance, good dynamic coating ability, and low viscosity. single-base resolutions of dsdna fragments (r-s = 0.92 for 123/124 bp) and ssdna fragments (r-s = 0.65 for 123/124 base, r-s = 0.48 for 309/310 base) were achieved by using the quasi-ipn of poly(tris-a)/pvp (2% + 2%) solution in a 31 cm effective length linear polyacrylamide (lpa)-coated column. single-base separation of dsdna fragments (r,, = 0.92 for 123/124 bp) was also obtained within 28 min in a 46.7 cm effective length bare column at higher 160 v/cm electric field strength by using the same quasi-ipn solution. the rsd of the migration time measured for each dna fragments was less than 1.5% in the bare column for nine continuous runs. the effects of temperature and electric field strength on the dna separation were also investigated. |
| WOS关键词 | LINEAR POLYACRYLAMIDE SOLUTIONS ; ACRYLOYL TRIS GELS ; RESTRICTION FRAGMENTS ; 1000 BASES ; POLY(N,N-DIMETHYLACRYLAMIDE) ; RESOLUTION ; POLYMERS ; VISCOSITY |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000237685600009 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379574 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Xu, X |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Organ Chem, Lab Analyt Chem, State Key Lab Organomet Chem, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Q,Xu, X,Dai, LX. A new quasi-interpenetrating network formed by poly(n-acryloyl-tris(hydroxymethyl)aminomethane and polyvinylpyrrolidone: separation matrix for double-stranded dna and single-stranded dna fragments by capillary electrophoresis with uv detection[J]. Electrophoresis,2006,27(9):1749-1757. |
| APA | Wang, Q,Xu, X,&Dai, LX.(2006).A new quasi-interpenetrating network formed by poly(n-acryloyl-tris(hydroxymethyl)aminomethane and polyvinylpyrrolidone: separation matrix for double-stranded dna and single-stranded dna fragments by capillary electrophoresis with uv detection.Electrophoresis,27(9),1749-1757. |
| MLA | Wang, Q,et al."A new quasi-interpenetrating network formed by poly(n-acryloyl-tris(hydroxymethyl)aminomethane and polyvinylpyrrolidone: separation matrix for double-stranded dna and single-stranded dna fragments by capillary electrophoresis with uv detection".Electrophoresis 27.9(2006):1749-1757. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。