Cloning and characterization of a second form of the rice adenine phosphoribosyl transferase gene (osapt2) and its association with tgms
文献类型：期刊论文
| 作者 | Zhou, CJ; Li, J; Zou, JC; Liang, FS; Ye, CJ; Jin, DM; Weng, ML; Wang, B |
| 刊名 | Plant molecular biology
 |
| 出版日期 | 2006-02-01 |
| 卷号 | 60期号:3页码:365-376 |
| 关键词 | Adenine phosphoribosyl transferase (aprt) Arabidopsis thaliana Oryza sativa Thermo-sensitive genic male sterility (tgms) |
| ISSN号 | 0167-4412 |
| DOI | 10.1007/s11103-005-4208-5 |
| 通讯作者 | Wang, b() |
| 英文摘要 | A rice gene, osapt2, which encodes a putative adenine phosphoribosyl transferase (aprt), was cloned and characterized. analysis of the cdna and genomic sequences revealed seven exons and six introns in the osapt2. the deduced amino acid sequence of osapt2 is highly homologous to those of previously isolated aprts. rt-pcr analysis indicated that the osapt2 transcript in the young panicles of 'annong s-1' is down-regulated at 29 degrees c, the critical temperature for induction of 'annong s-1' fertility conversion. since the panicle is likely the thermo-sensitive organ at the early stages of pollen fertility alternation, the observed heat-induced change in the osapt2 expression pattern in young panicles may mediate, at least in part, thermo-sensitive genic male sterility (tgms) in 'annong s-1'. an antisense strategy was used to suppress the expression of the osapt2 homolog in arabidopsis, and the obtained homozygous transgenic plants contained lower amp content, displayed lower pollen germination rates and exhibited some abnormalities in leaf phenotypes and flowering timing. these data suggest that osapt2 is likely to be involved in tgms in the rice line 'annong s-1'. |
| WOS关键词 | ARABIDOPSIS-THALIANA ; PURINE METABOLISM ; HIGHER-PLANTS ; CYTOKININ METABOLISM ; TOMATO PLANT ; SUPPRESSION ; PETUNIA ; MUTANT ; APRT |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS类目 | Biochemistry & Molecular Biology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000235750500003 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379575 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, B |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050016, Hebei, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, CJ,Li, J,Zou, JC,et al. Cloning and characterization of a second form of the rice adenine phosphoribosyl transferase gene (osapt2) and its association with tgms[J]. Plant molecular biology,2006,60(3):365-376. |
| APA | Zhou, CJ.,Li, J.,Zou, JC.,Liang, FS.,Ye, CJ.,...&Wang, B.(2006).Cloning and characterization of a second form of the rice adenine phosphoribosyl transferase gene (osapt2) and its association with tgms.Plant molecular biology,60(3),365-376. |
| MLA | Zhou, CJ,et al."Cloning and characterization of a second form of the rice adenine phosphoribosyl transferase gene (osapt2) and its association with tgms".Plant molecular biology 60.3(2006):365-376. |
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来源：中国科学院大学
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