Effects of codon modification on human bmp2 gene expression in tobacco plants
文献类型：期刊论文
| 作者 | Suo, GL; Chen, B; Zhang, JY; Duan, ZY; He, ZQ; Yao, W; Yue, CY; Dai, JW |
| 刊名 | Plant cell reports
 |
| 出版日期 | 2006-07-01 |
| 卷号 | 25期号:7页码:689-697 |
| 关键词 | Codon optimization Codon usage Human bmp2 Transgenic tobacco |
| ISSN号 | 0721-7714 |
| DOI | 10.1007/s00299-006-0133-6 |
| 通讯作者 | Dai, jw() |
| 英文摘要 | Bone morphogenetic protein 2 (bmp2) has great potential in therapeutic applications. we are working on generating transgenic plants as a bioreactor to produce bmp2. we have studied the effects of codon optimization on the expression of human bmp2 (hbmp2) in tobacco plants. three modified hbmp2 genes were transformed into tobacco under the control of either cauliflower mosaic virus 35s (camv35s) promoter or double-camv35s promoter plus alfalfa mosaic virus (amv) enhancer. the fused beta-glucuronidase (gus) reporter gene was used to facilitate the assay of protein expression. the results indicated that codon optimization could increase the protein expression level obviously under camv35s promoter. however, under relatively stronger initiation condition (double-camv35s promoter plus amv enhancer), only the gene with the lowest degree of codon optimization could increase the protein expression level. our findings suggest that the action of codon optimization may be influenced by the factors of promoter strength and a+t content in tobacco plants. |
| WOS关键词 | BONE MORPHOGENETIC PROTEIN-2 ; MESSENGER-RNA STABILITY ; HIGH-LEVEL EXPRESSION ; BIAS ; VIRUS ; CELLS ; USAGE ; SITE ; DNA |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000238317700009 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379583 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Dai, JW |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Ctr Mol Dev Biol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China 3.China Three Gorges Univ, Biotechnol Res Ctr, Yichang 443002, Peoples R China |
| 推荐引用方式 GB/T 7714 | Suo, GL,Chen, B,Zhang, JY,et al. Effects of codon modification on human bmp2 gene expression in tobacco plants[J]. Plant cell reports,2006,25(7):689-697. |
| APA | Suo, GL.,Chen, B.,Zhang, JY.,Duan, ZY.,He, ZQ.,...&Dai, JW.(2006).Effects of codon modification on human bmp2 gene expression in tobacco plants.Plant cell reports,25(7),689-697. |
| MLA | Suo, GL,et al."Effects of codon modification on human bmp2 gene expression in tobacco plants".Plant cell reports 25.7(2006):689-697. |
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来源：中国科学院大学
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