Ahssk1, a novel skp1-like protein that interacts with the s-locus f-box protein slf
文献类型：期刊论文
| 作者 | Huang, J; Zhao, L; Yang, QY; Xue, YB |
| 刊名 | Plant journal
 |
| 出版日期 | 2006-06-01 |
| 卷号 | 46期号:5页码:780-793 |
| 关键词 | Self-incompatibility Skp1 F-box Slf Ssk1 Antirrhinum |
| ISSN号 | 0960-7412 |
| DOI | 10.1111/j.1365-313x.2006.02735.x |
| 通讯作者 | Xue, yb() |
| 英文摘要 | The s-locus f-box (slf/sfb) protein, recently identified as the pollen determinant of s-rnase-based self-incompatibility (si) in solanaceae, scrophulariaceae and rosaceae, has been proposed to serve as the subunit of an scf (skp1-cul1-f-box) ubiquitin ligase and to target its pistil counterpart s-rnase during the si response. however, the underlying mechanism is still in dispute, and the putative slf-binding skp1-equivalent protein remains unknown. here, we report the identification of ahssk1, antirrhinum hispanicum slf-interacting skp1-like1, using a yeast two-hybrid screen against a pollen cdna library. gst pull-down assays confirmed the ssk1-slf interaction, and showed that ahssk1 could connect ahslf to a cul1-like protein. ahssk1, despite having a similar secondary structure to other skp1-like proteins, appeared quite distinctive in sequence and unique in a phylogenetic analysis, in which no ssk1 ortholog could be predicted in the sequenced genomes of arabidopsis and rice. thus, our results suggest that the pollen-specific ssk1 could be recruited exclusively as the adaptor of putative scfslf in those plants with s-rnase-based si, providing an important clue to dissecting the function of the pollen determinant. |
| WOS关键词 | SCF UBIQUITIN-LIGASE ; GAMETOPHYTIC SELF-INCOMPATIBILITY ; ARABIDOPSIS-THALIANA ; TRANSCRIPTIONAL ANALYSIS ; CAENORHABDITIS-ELEGANS ; PROTEASOME PATHWAY ; SEQUENCE ALIGNMENT ; ALLELIC DIVERSITY ; POLLEN FUNCTION ; AUXIN RECEPTOR |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000237634900006 |
| 出版者 | BLACKWELL PUBLISHING |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379609 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Xue, YB |
| 作者单位 | 1.Chinese Acad Sci, Mol & Dev Biol Lab, Inst Genet & Dev Biol, Beijing 100080, Peoples R China 2.Natl Ctr Plant Gene Res, Beijing 100080, Peoples R China 3.Grad Univ, Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, J,Zhao, L,Yang, QY,et al. Ahssk1, a novel skp1-like protein that interacts with the s-locus f-box protein slf[J]. Plant journal,2006,46(5):780-793. |
| APA | Huang, J,Zhao, L,Yang, QY,&Xue, YB.(2006).Ahssk1, a novel skp1-like protein that interacts with the s-locus f-box protein slf.Plant journal,46(5),780-793. |
| MLA | Huang, J,et al."Ahssk1, a novel skp1-like protein that interacts with the s-locus f-box protein slf".Plant journal 46.5(2006):780-793. |
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来源：中国科学院大学
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