Induction of apoptosis in k562 cells by jolkinolide b
文献类型：期刊论文
| 作者 | Luo, Huiying; Wang, Aiqin |
| 刊名 | Canadian journal of physiology and pharmacology
 |
| 出版日期 | 2006-10-01 |
| 卷号 | 84期号:10页码:959-965 |
| 关键词 | Jolkinolide b K562 cells Apoptosis |
| ISSN号 | 0008-4212 |
| DOI | 10.1139/y06-045 |
| 通讯作者 | Wang, aiqin(aqwang@lzb.ac.cn) |
| 英文摘要 | Jolkinolide b, a bioactive diterpene isolated from the roots of euphorbia fischeriana steud, has various biological and pharmacological properties. in this study, the cytotoxicity of highly purified jolkinolide b was tested in human chronic myeloid leukemia (k562) and 2 other cell lines (human esophageal carcinoma eca-109 and human hepatoma hepg2). the results indicate a significant decrease in the proliferation of all the 3 cell lines when treated with jol-kinolide b for 24 h; the ic50 value of cytotoxicity was 12.1 mu g/ml (for k562 cells), > 50.0 mu g/ml (for hepg2 cells), and 23.7 mu g/ml (for eca-109 cells). further study of k562 cells involving fluorescence and transmission electron microscopy revealed characteristic apoptotic features, such as cell shrinkage, membrane blebbing, loss of microvilli and nuclear condensation. agarose electrophoresis of genomic dna showed a typical fragmentation pattern for apoptotic cells. a kinetic cell-cycle analysis demonstrated that the cell cycle was arrested in the g i phase. all these results suggest, that the anti-proliferation effect of jol-kinolide b on k562 cells is achieved by arresting the cell cycle in the g i phase and subsequently inducing apoptosis. |
| WOS关键词 | EUPHORBIA-FISCHERIANA ; AGENTS ; CYCLE ; PROLIFERATION ; DITERPENOIDS ; MEDICINE ; ARREST ; LINES |
| WOS研究方向 | Pharmacology & Pharmacy ; Physiology |
| WOS类目 | Pharmacology & Pharmacy ; Physiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000243731100001 |
| 出版者 | NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379631 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, Aiqin |
| 作者单位 | 1.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luo, Huiying,Wang, Aiqin. Induction of apoptosis in k562 cells by jolkinolide b[J]. Canadian journal of physiology and pharmacology,2006,84(10):959-965. |
| APA | Luo, Huiying,&Wang, Aiqin.(2006).Induction of apoptosis in k562 cells by jolkinolide b.Canadian journal of physiology and pharmacology,84(10),959-965. |
| MLA | Luo, Huiying,et al."Induction of apoptosis in k562 cells by jolkinolide b".Canadian journal of physiology and pharmacology 84.10(2006):959-965. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。