Involvement of smad4, but not of smad2, in transforming growth factor-beta1-induced trophoblast expression of matrix metalloproteinase-2
文献类型：期刊论文
| 作者 | Lin, HY; Yang, Q; Wang, HM; Qi, JG; Zhang, H; Wang, HX; Tsang, BK; Zhu, C |
| 刊名 | Frontiers in bioscience
 |
| 出版日期 | 2006 |
| 卷号 | 11页码:637-646 |
| 关键词 | Transcription factor Cytokine Matrix Smad Tgf-beta Mmp Retrovirus Trophoblast |
| ISSN号 | 1093-9946 |
| 通讯作者 | Zhu, c() |
| 英文摘要 | Matrix metalloproteinases (mmps) play crucial roles in extravillous trophoblast invasion. in the present study, we examined the possible role of smad4 and smad2 in transforming growth factor (tgf)-beta1-induced mmp-2 expression, using the well-established invasive extravillous trophoblast cell line htr-8/svneo. recombinant sense smad4 or smad2 retroviral vectors were constructed by inserting full-length smad4 or smad2 cdna into plxsn retroviral vector. stable pt67 packaging cell clones were isolated and viral supernatants were used to infect htr8/svneo cells. effects of retroviral expression of smad4 and smad2 on tgf-beta1-regulated mmp-2 expression were assessed by semi-quantitative reverse transcription-polymerase chain reaction and gelatin zymography. the results showed that over-expression of smad4 augmented mmp-2 mrna abundance and the secretion of pro-mmp2, and mimicked the inductive effect of tgf-beta1 on the production of mmp-2. however, retrovirus-mediated sense smad2 gene transfer had no effect. these findings suggest that smad4, but not smad2, mediates tgf-beta1-induced mmp-2 expression in invasive extravillous trophoblasts. |
| WOS关键词 | FACTOR-BETA ; TGF-BETA ; TRANSCRIPTIONAL ACTIVATION ; SIGNALING PATHWAYS ; EARLY-PREGNANCY ; DNA-BINDING ; MH1 DOMAIN ; CELLS ; FIBROBLASTS ; INHIBITORS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
| WOS类目 | Biochemistry & Molecular Biology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000232528000049 |
| 出版者 | FRONTIERS IN BIOSCIENCE INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379637 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhu, C |
| 作者单位 | 1.Chinese Acad Sci, Inst Zool, Reprod Biol Lab, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Univ Ottawa, Reprod Biol Unit, Ottawa, ON K1Y 4E9, Canada 4.Univ Ottawa, Div Reprod Med, Ottawa, ON K1Y 4E9, Canada 5.Univ Ottawa, Div Gynaecol Oncol, Dept Obstet & Gynaecol, Ottawa, ON K1Y 4E9, Canada 6.Ottawa Hlth Res Inst, Hormones Growth & Dev Program, Ottawa, ON K1Y 4E9, Canada 7.Natl Inst Viral Dis Control & Prevent, Chinese Ctr Dis Control & Prevent, Beijing 100052, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, HY,Yang, Q,Wang, HM,et al. Involvement of smad4, but not of smad2, in transforming growth factor-beta1-induced trophoblast expression of matrix metalloproteinase-2[J]. Frontiers in bioscience,2006,11:637-646. |
| APA | Lin, HY.,Yang, Q.,Wang, HM.,Qi, JG.,Zhang, H.,...&Zhu, C.(2006).Involvement of smad4, but not of smad2, in transforming growth factor-beta1-induced trophoblast expression of matrix metalloproteinase-2.Frontiers in bioscience,11,637-646. |
| MLA | Lin, HY,et al."Involvement of smad4, but not of smad2, in transforming growth factor-beta1-induced trophoblast expression of matrix metalloproteinase-2".Frontiers in bioscience 11(2006):637-646. |
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来源：中国科学院大学
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