Trimeric structure of the wild soluble chloride intracellular ion channel clic4 observed in crystals
文献类型：期刊论文
| 作者 | Li, YF; Li, DF; Zeng, ZH; Wang, DC |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2006-05-19 |
| 卷号 | 343期号:4页码:1272-1278 |
| 关键词 | Chloride intracellular ion channel Clic4 Crystal structure Trimeric structure |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2006.03.099 |
| 通讯作者 | Wang, dc() |
| 英文摘要 | The crystal structure of a wild type of the human soluble chloride intracellular ion channel clic4 (wclic4) has been determined at a resolution of 2.2 angstrom. the structure shows a homotrimer in an asymmetric unit, which is first observed in clics. the assembly of the trimer takes a unique triple interaction mode between three monomers with a hydrogen-bond network and hydrophobic contacts. through such complicated interactions, the homotrimer of wclic4 is firmly stabilized. the structure shows an oligomeric mode with a unique assembly mechanism by which the oligomerization of clic4 can be performed without any intramolecular disulfide bond formation. it indicated a possibility that clic4 may take a unique structural organization distinct from clic1 for docking with lipid bilayers. in addition, the structure shows distinct conformational states of the h2 region for respective monomers of the trimer, which reveal an intrinsic conformational susceptibility for this significant region in the structural transition. (c) 2006 elsevier inc. all rights reserved. |
| WOS关键词 | MOLECULAR-CLONING ; PROTEIN ; EXPRESSION ; MEMBER ; FAMILY ; NCC27 ; GENE ; FORM ; P64 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000237269000041 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379659 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, DC |
| 作者单位 | 1.Chinese Acad Sci, Natl Lab Biomacromol, Inst Biophys, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, YF,Li, DF,Zeng, ZH,et al. Trimeric structure of the wild soluble chloride intracellular ion channel clic4 observed in crystals[J]. Biochemical and biophysical research communications,2006,343(4):1272-1278. |
| APA | Li, YF,Li, DF,Zeng, ZH,&Wang, DC.(2006).Trimeric structure of the wild soluble chloride intracellular ion channel clic4 observed in crystals.Biochemical and biophysical research communications,343(4),1272-1278. |
| MLA | Li, YF,et al."Trimeric structure of the wild soluble chloride intracellular ion channel clic4 observed in crystals".Biochemical and biophysical research communications 343.4(2006):1272-1278. |
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来源：中国科学院大学
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