Differential regulation of chromatin structure of the murine 3 ' igh enhancer and igg2b germline promoter in response to lipopolysaccharide and cd40 signaling
文献类型：期刊论文
| 作者 | Qin, XC; Tang, H |
| 刊名 | Molecular immunology
 |
| 出版日期 | 2006-03-01 |
| 卷号 | 43期号:8页码:1211-1220 |
| 关键词 | Chromatin remodeling Histone acetylation Igh Class switch recombination |
| ISSN号 | 0161-5890 |
| DOI | 10.1016/j.molimm.2005.07.016 |
| 通讯作者 | Tang, h() |
| 英文摘要 | Class switch recombination (csr) of murine immunoglobulin heavy chain (igh) is controlled by germline transcription-coupled modification of the accessibility of the highly repetitive switch regions (s) located upstream of the constant region genes. activation of the 3' igh enhancer (ye) is believed to regulate csr during b cell terminal differentiation, although the detailed molecular mechanism remains unclear. here, we show that baf57 and brg1 two essential subunits of murine swi/snf complex, differentially associate with the dnase i hypersensitive region hs 1/2 of 3'e and the igg2b germline promoter in response to lps activation or cd40 engagement. both lps and cd40 signaling cause swi/snf complex to dissociate from hs 1/2 and associate with their responsive igg2b germline promoter, suggesting the potential fluidity of chromatin structure and specific regulatory mode for the atp-dependent chromatin remodeler during csr. more interesting. increase in histone acetylation is either inverse or parallel with the action of swi/snf complex at hs 1/2 enhancer or lgg2b germline promoter, respectively. chromatin immunoprecipitation experiments show that alteration of histone h3 and h4 acetylation has overall similarities in response to lps and cd40 signaling, with h3 hyperacetylated and h4 hypoacetylated at the hs 1/2 enhancer and reversed modification patterns at the igg2b germline promoter. finally, the specificity of lps and cd40 signaling in control of csr could be partially coded by the specific acetylation marking of h3 and h4. our results further strengthen the notion that chromatin remodeling plays a critical role in csr. (c) 2005 elsevier ltd. all rights reserved. |
| WOS关键词 | LOCUS-CONTROL REGION ; HEAVY-CHAIN LOCUS ; HISTONE ACETYLATION ; TRANSCRIPTIONAL ACTIVATOR ; REPRESSES TRANSCRIPTION ; SWI/SNF COMPLEXES ; HS1,2 ENHANCER ; B-CELLS ; EXPRESSION ; NUCLEOSOME |
| WOS研究方向 | Biochemistry & Molecular Biology ; Immunology |
| WOS类目 | Biochemistry & Molecular Biology ; Immunology |
| 语种 | 英语 |
| WOS记录号 | WOS:000235726900015 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379692 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Tang, H |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, Ctr Mol Microbiol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Inst Biophys, Ctr Infect & Immun, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qin, XC,Tang, H. Differential regulation of chromatin structure of the murine 3 ' igh enhancer and igg2b germline promoter in response to lipopolysaccharide and cd40 signaling[J]. Molecular immunology,2006,43(8):1211-1220. |
| APA | Qin, XC,&Tang, H.(2006).Differential regulation of chromatin structure of the murine 3 ' igh enhancer and igg2b germline promoter in response to lipopolysaccharide and cd40 signaling.Molecular immunology,43(8),1211-1220. |
| MLA | Qin, XC,et al."Differential regulation of chromatin structure of the murine 3 ' igh enhancer and igg2b germline promoter in response to lipopolysaccharide and cd40 signaling".Molecular immunology 43.8(2006):1211-1220. |
入库方式： iSwitch采集
来源：中国科学院大学
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