Efficient agrobacterium-mediated transformation of rice by phosphomannose isomerase/mannose selection
文献类型：期刊论文
| 作者 | Ding Zai-Song; Zhao Ming; Jing Yu-Xiang; Li Liang-Bi; Kuang Ting-Nin |
| 刊名 | Plant molecular biology reporter
 |
| 出版日期 | 2006-09-01 |
| 卷号 | 24期号:3-4页码:295-303 |
| 关键词 | Agrobacterium-mediated transformation Mannose selection Transgenic rice |
| ISSN号 | 0735-9640 |
| 通讯作者 | Jing yu-xiang(yxjing@ibcas.ac.cn) |
| 英文摘要 | A positive selection system was developed for agrobacterium-mediated transformation of rice that does not use toxic compounds such as antibiotics or herbicides. the selection system is based on the escherichia coli phosphomannose isomerase (pmi) gene as a selectable marker and mannose as the selective agent. only transgenic plants were able to metabolize mannose into a usable source of carbon, fructose. selection was achieved using a combination of mannose and sucrose at 10 g/l and 5 g/l, respectively. transgenic rice plants were produced efficiently in japanica rice variety zhonghua 8, with transformation frequency of 16.5%, which was slightly lower than that achieved by hygromycin selection. |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS类目 | Biochemical Research Methods ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000202988000002 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379729 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Jing Yu-Xiang |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding Zai-Song,Zhao Ming,Jing Yu-Xiang,et al. Efficient agrobacterium-mediated transformation of rice by phosphomannose isomerase/mannose selection[J]. Plant molecular biology reporter,2006,24(3-4):295-303. |
| APA | Ding Zai-Song,Zhao Ming,Jing Yu-Xiang,Li Liang-Bi,&Kuang Ting-Nin.(2006).Efficient agrobacterium-mediated transformation of rice by phosphomannose isomerase/mannose selection.Plant molecular biology reporter,24(3-4),295-303. |
| MLA | Ding Zai-Song,et al."Efficient agrobacterium-mediated transformation of rice by phosphomannose isomerase/mannose selection".Plant molecular biology reporter 24.3-4(2006):295-303. |
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来源：中国科学院大学
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